13
13. For conformity evaluation calculate the random error of the
measurement:
as standard deviation s = ∑(V
i
- v )
2
n = number of
measurement (10)
n - 1
or as coeffi cient of variation CV = 100
s/v
14. Compare the systematic error (inaccuracy) and random error
(imprecision) with the values in the performance specifi cations
- (p. 49) or the specifi cations of your own laboratory.
If the results fall within the specifi cations, the pipette is ready
for use. Otherwise check both systematic and random errors
and, when necessary, proceed to the recalibration procedure
(Chapter 12.2).
Note: Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the
mean of the dispensed volume. (ISO 8655-1.)
Note: Biohit specifi cations are achieved in strictly controlled condi-
tions (ISO 8655-6). The user should establish own specifi cations
based on the fi eld of use and the accuracy requirements placed on
the pipettor (ISO8655-1).
Table 1
Note: This method is based on ISO 8655.
Z-values (μl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0 1.0028 1.0028 1.0029 1.0029
20.5 1.0029 1.0029 1.0030 1.0030
21.0 1.0030 1.0031 1.0031 1.0031
21.5 1.0031 1.0032 1.0032 1.0032
22.0 1.0032 1.0033 1.0033 1.0033
22.5 1.0033 1.0034 1.0034 1.0034
23.0 1.0034 1.0035 1.0035 1.0036
23.5 1.0036 1.0036 1.0036 1.0037
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