Biorep Technologies PERI-4.2 - Table of Contents

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TABLE OF CONTENTS

1 GETTING STARTED ................................................................................................................................................................. 3

1.1 SYMBOLS USED IN THIS MANUAL ........................................................................................................................................................... 3

1.2 MACHINE INFORMATION ......................................................................................................................................................................... 3

1.3 CONTACT INFORMATION ......................................................................................................................................................................... 3

1.4 SAFETY INFORMATION ............................................................................................................................................................................ 4

1.5 SITE PREPARATION ................................................................................................................................................................................. 5

1.6 PACKING LIST ......................................................................................................................................................................................... 5

2 INTRODUCTION ........................................................................................................................................................................ 6

3 MACHINE PARTS IDENTIFICATION .................................................................................................................................. 7

4 ACCESSORIES ............................................................................................................................................................................ 9

4.1 TUBING SET ............................................................................................................................................................................................ 9

4.2 PERIFUSION CHAMBER ............................................................................................................................................................................ 9

4.3 PERIFUSION CHAMBER AUXILIARY RACK .............................................................................................................................................. 10

4.4 PERIFUSION NOZZLES ........................................................................................................................................................................... 10

4.5 COLLECTION WELL-PLATES................................................................................................................................................................... 10

4.6 BIO-GEL P-4 ......................................................................................................................................................................................... 10

4.7 FIBERGLASS PRE-FILTERS ...................................................................................................................................................................... 10

5 PERIFUSION SYSTEM OVERVIEW .................................................................................................................................... 11

5.1 BASIC PERIFUSION SCHEMATIC ............................................................................................................................................................. 11

5.2 VALVE MANIFOLD ................................................................................................................................................................................ 12

5.3 PERISTALTIC PUMP ................................................................................................................................................................................ 14

5.4 PERIFUSION CHAMBER .......................................................................................................................................................................... 14

5.5 COLLECTION TRAY ................................................................................................................................................................................ 15

6 PERIFUSION SYSTEM BASICS ............................................................................................................................................ 16

6.1 POWER-UP ............................................................................................................................................................................................ 16

6.2 LOGGING IN .......................................................................................................................................................................................... 17

6.3 MAIN MENU.......................................................................................................................................................................................... 18

6.4 TEST PAGE ............................................................................................................................................................................................ 19

6.5 SETTINGS .............................................................................................................................................................................................. 21

7 PERIFUSION OPERATION (PROTOCOL WIZARD) ...................................................................................................... 22

7.1 CHAMBER POSITIONING......................................................................................................................................................................... 22

7.2 PROTOCOL............................................................................................................................................................................................. 22

7.3 SYSTEM SETUP ...................................................................................................................................................................................... 23

7.4 CHAMBER SETUP .................................................................................................................................................................................. 24

7.5 PERIFUSION TUBING SETUP................................................................................................................................................................... 24

7.6 PRIMING ................................................................................................................................................................................................ 25

7.7 CELL LOADING ...................................................................................................................................................................................... 25

7.8 CHAMBER POSITIONING......................................................................................................................................................................... 26

7.9 TEMPERATURE CONTROL ...................................................................................................................................................................... 26

7.10 PROTOCOL EXECUTION.......................................................................................................................................................................... 27

7.11 PROTOCOL EXAMPLES .......................................................................................................................................................................... 29

8 FINALIZING THE EXPERIMENT ........................................................................................................................................ 31

8.1 COLLECTING THE CELLS ........................................................................................................................................................................ 31

8.2 CLEANING THE SYSTEM ......................................................................................................................................................................... 32

9 REPORT ...................................................................................................................................................................................... 32

10 CUSTOMER SERVICE ............................................................................................................................................................ 33

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