35
Bead Suspension Preparation
Add beads to 5ml mark of a 50ml conical
Add 40ml of buffer solution.
Prepare bead-buffer suspension at least one day before the experiment to
allow beads to absorb buffer.
Keep the bead-buffer suspension refrigerated (4℃)
Bead suspension may be kept refrigerated for up to 4 weeks
Bead Suspension Use
Follow these instructions before using the bead suspension in a perifusion chamber preparation:
Warm bead suspension to 37℃ using an incubator or water bath. Do not add cold bead
suspension to cells, since this may adversely affect your experiment.
Agitate conical thoroughly before pipetting bead suspension to add into perifusion columns. This
ensures that approximately even number of beads are present in each column.
To Previous Page