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Biotek Synergy HT User Manual

Biotek Synergy HT
240 pages
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Operator’s Manual
Multi-Mode Microplate Reader
Synergy
HT

Table of Contents

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Biotek Synergy HT Specifications

General IconGeneral
BrandBiotek
ModelSynergy HT
CategoryLaboratory Equipment
LanguageEnglish

Summary

Notices

BioTek Instruments, Inc. Contact Information

Contact details for BioTek Instruments, Inc. including address and general information.

Trademarks and Copyright

Information regarding registered trademarks and copyright of BioTek Instruments, Inc.

Restrictions and Liabilities

Details on limitations and responsibilities regarding the use of the document and product.

Contact Information

Customer Service and Sales Contact

Provides contact details for customer service and sales inquiries.

Service/TAC Contact Information

Contact information for the Technical Assistance Center (TAC) for service.

European Coordination Center Contact

Contact details for the European representative and coordination center.

Revision History

Document Conventions

Intended Use Statement and Quality Control

Intended Use Statement

Defines the purpose and scope of the Synergy HT instrument's use.

Quality Control Guidelines

Recommendations for maintaining good laboratory practice for assay accuracy.

Warranty and Product Registration

Information on product warranty and the process for registration.

Safety Warnings and Precautions

General Warnings

Important warnings related to instrument operation and environmental conditions.

Hazards and Precautions

Detailed hazards and necessary precautions for safe instrument operation.

Electromagnetic Interference and Susceptibility

Information on EMC compliance and potential interference.

User Safety Standards

Details on the safety standards the device has been tested against.

Safety Symbols

Chapter 1: Introduction

Synergy HT Reader Overview

Introduction to the Synergy HT Multi-Mode Microplate Reader.

Key Features

Highlights of the Synergy HT's main functionalities and capabilities.

Package Contents

List of items included in the Synergy HT package.

Optional Accessories

List of optional accessories available for the Synergy HT.

Product Support and Service

Information on obtaining technical and application support.

Chapter 2: Installation

Product Registration Process

Instructions for registering your Synergy HT product.

Unpacking and Inspecting the Reader

Steps for safely unpacking and inspecting the Synergy HT reader.

Removing the Shipping Panel

Procedure for removing the reader's shipping panel before operation.

Removing the Microplate Carrier Shipping Screw

Instructions for removing the shipping screw for the microplate carrier.

Installing the Fluorescence Lamp Assembly

Steps to install the fluorescence lamp assembly into the instrument.

Selecting an Appropriate Location

Guidelines for choosing a suitable installation environment for the reader.

Connecting the Power Supply

Procedure for connecting the instrument's external power supply.

Unpacking and Inspecting the Dispense Module

Steps to unpack and inspect the dispense module for injector models.

Installing the Dispense Module

Instructions for installing the dispense module onto the Synergy HT.

Connecting the Host Computer

Steps for connecting the Synergy HT to a host computer.

Installing the Software on the Host Computer

Guide for installing Gen5 or KC4 software on the host computer.

Turning on the Reader

Procedure for powering on the Synergy HT instrument.

Establishing Communication

Steps to establish communication between the reader and software.

Running a System Test

Procedure for running the system test to verify instrument setup.

Testing the Injector System

Procedure for testing the dispense module's injector system.

Operational and Performance Qualification

Information on qualifying instrument performance post-installation.

Repackaging and Shipping Instructions

Guidance on properly repackaging the instrument for shipment.

Chapter 3: Getting Started

Key Components Overview

Description of the Synergy HT's main components and their functions.

Lamp Assembly and Filter Wheel Access

How to access the lamp assembly and filter wheels for inspection or cleaning.

Excitation and Emission Filter Wheels

Details on the excitation and emission filter wheels and their orientation.

Installing the Time-Resolved Fluorescence Cartridge

Instructions for installing the TR cartridge for time-resolved fluorescence assays.

Configuring for Luminescence Measurements

Steps to configure the system for optimal luminescence measurements.

The External Dispense Module

Description of the external dispense module components and function.

Gen5 Software Operations

Guide to using Gen5 software for reader control and data analysis.

KC4 Software Operations

Guide to using KC4 software for reader control and data analysis.

Controlling the Dispense Module (Gen5)

Using Gen5 software for dispense module initialization and priming.

Controlling the Dispense Module (KC4)

Using KC4 software for dispense module initialization and priming.

Recommendations for Optimum Performance

Tips and recommendations to achieve the best performance from the instrument.

Chapter 4: Instrument Qualification

Instrument Qualification Overview

Introduction to the procedures for qualifying instrument performance.

IQ, OQ, and PQ Qualification Procedures

Details on Installation, Operational, and Performance Qualification steps.

Recommended Qualification Schedule

Factory-recommended intervals for performing qualification tests.

System Test Procedure

Steps and description for performing the System Test.

Absorbance Plate Test

Procedure for testing absorbance accuracy, linearity, and repeatability.

Absorbance Liquid Tests

Procedures for verifying performance with liquid samples.

Fluorescence Tests

Procedures for testing instrument performance in fluorescence mode.

Dispense Module Tests

Tests to verify the performance of the dispense module for injector models.

Dispense Accuracy & Precision Tests Worksheet

Worksheet for recording dispense accuracy and precision test results.

Chapter 5: Preventive Maintenance

Recommended Maintenance Schedule

Schedule for routine preventive maintenance tasks.

Warnings and Precautions for Maintenance

Important safety warnings before performing maintenance procedures.

Cleaning Exposed Surfaces

Procedure for cleaning the instrument's external surfaces.

Inspecting and Cleaning Filters

Instructions for inspecting and cleaning excitation and emission filters.

Flushing and Purging the Fluid Path

Procedure for flushing and purging the fluid path in injector models.

Running a Dispense Protocol for Observation

Optional protocol to visually inspect dispensing accuracy after maintenance.

Emptying and Cleaning the Tip Priming Trough

Procedure for emptying and cleaning the tip priming trough.

Cleaning the Priming Plate

Instructions for cleaning the priming plate.

Cleaning Internal Components

Detailed procedures for cleaning internal instrument components.

Reassembling Components

Steps for reassembling the instrument after cleaning internal parts.

Performance Check After Maintenance

Procedure to verify instrument function after reassembly.

Appendix A: Decontamination Procedures

Purpose of Decontamination

Explanation of why decontamination is necessary for laboratory instruments.

Required Materials for Decontamination

List of materials needed for decontamination procedures.

Decontamination Procedure for Models without Injectors

Steps for decontaminating Synergy HT models without injectors.

Routine Decontamination for Injector Models

Routine decontamination steps for Synergy HT models with injectors.

Alternate Decontamination for Injector Models

Alternative decontamination procedure for injector models due to system failure.

Appendix B: Computer Control

Appendix C: Error Codes

General Error Code Information

Explanation of error code format and general troubleshooting steps.

Fatal Errors

List and description of fatal errors requiring immediate attention.

Non-Fatal Errors: Home Sensor Initial Find

Errors related to the home sensor transition for various axes.

Non-Fatal Errors: Home Sensor Verification

Errors indicating failure to verify home sensor position.

Non-Fatal Errors: Saturation

Errors indicating unexpected saturation conditions in measurement channels.

Non-Fatal Errors: Absorbance Reader Noise

Errors indicating elevated noise levels in absorbance measurement channels.

Non-Fatal Errors: Internal Self-Test

Errors detected during the instrument's internal self-tests.

Non-Fatal Errors: Other

List and description of miscellaneous non-fatal errors.

Appendix D: Specifications

Instrument Specifications Overview

General specifications for the Synergy HT instrument.

Microplate Compatibility

Details on supported microplate types and geometries.

Hardware and Environmental Specifications

Specifications for light sources, dimensions, weight, and environment.

Absorbance Specifications

Performance specifications for absorbance measurements.

Fluorescence Specifications

Performance specifications for fluorescence measurements.

Read Modes and Timing

Description of available read modes and timing parameters.

Optical Probes and Sensitivity

Details on optical probe sizes and sensitivity benchmarks.

Incubation and Shake Specifications

Specifications for the instrument's incubation and shaking features.

Injector Model Specifications

Specific performance specifications for injector models.

Appendix E: Instrument Dimensions for Robotic Interface

Instrument Dimensions for Robotic Integration

Information on instrument dimensions for robotic system setup.

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