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Biotek ELx800 User Manual

Biotek ELx800
218 pages
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Operator’s Manual
Absorbance Microplate Reader
ELx800

Table of Contents

Questions and Answers:

Biotek ELx800 Specifications

General IconGeneral
TypeMicroplate Reader
Detection MethodAbsorbance
Wavelength Range400-750 nm
DetectorSilicon Photodiode
Wavelength SelectionFilters
Plate Format6, 12, 24, 48, 96, 384 well plates
SoftwareGen5
Light SourceTungsten-Halogen Lamp
ShakingYes
InterfaceUSB
Dimensions (W x D x H)33 x 43 x 23 cm

Summary

Chapter 1: Introduction

Hardware Features

Details the physical components and capabilities of the ELx800 reader.

Software Features

Outlines the functionalities and capabilities of the ELx800's onboard software.

Package Contents

Lists all items included in the ELx800 instrument package upon purchase.

Optional Accessories

Describes additional items available for purchase to enhance ELx800 functionality.

Specifications

Provides detailed technical specifications for the standard, UV, and NB models.

Product Support & Service

Information on obtaining technical assistance and support for the ELx800 instrument.

Chapter 2: Installation

Unpacking and Inspecting the ELx800™

Step-by-step guide for safely removing the ELx800 from its shipping container.

Setting Up the ELx800

Instructions for configuring the ELx800, including environment, power, and initial settings.

Connecting a Printer to the ELx800

Procedure for connecting a compatible printer to the ELx800 for report output.

Setting Up the Serial Port for Communications With Other Devices

Guide for establishing serial communication with external devices like PCs.

Installing Additional Filters

Instructions on how to install or change interference filters in the reader's filter wheel.

Checking the Reader’s Filter Table Setting

Procedure to ensure software filter table matches physical filter locations.

Repackaging and Shipping the ELx800

Guidelines for properly packing and preparing the ELx800 for shipment.

Chapter 3: Operation

ELx800 Front Panel

Overview of the ELx800's keypad and display interface for operation.

Overview

Introduction to the ELx800's operational capabilities and features.

Recommendations for Achieving Optimum Performance

Best practices and tips for maximizing the performance and accuracy of the ELx800.

System Startup

Steps and procedures for powering on the ELx800 and initiating its system test.

Main Menu

Navigation and access to all reader functions from the primary menu.

Define

Procedures for setting up new assays or modifying existing ones, including method, map, formula, and curve.

Reading a Microplate

Instructions on how to select an assay and perform a microplate read.

Printing Reports

Details on how to generate and print various types of reports from the ELx800.

Using the Utility Options

Configuration of date/time, data output, and read options for the ELx800.

Chapter 4: Instrument Qualification

Recommended Qualification Schedule

Defines the recommended intervals for instrument performance testing.

Qualification Procedures

Outlines the tests to confirm initial and ongoing instrument performance.

System Test

Verifies instrument performance by checking light levels, noise, and sensitivity.

Absorbance Plate Test

Confirms mechanical alignment, accuracy, linearity, and repeatability using a test plate.

Liquid Testing

Verifies instrument performance with liquid samples to detect optical defects.

Chapter 5: Maintenance and Decontamination

Maintenance

Importance of daily maintenance for instrument accuracy and longevity.

Routine Cleaning Procedure

Step-by-step instructions for cleaning the ELx800 instrument surfaces.

Filter Storage and Handling

Guidelines for proper storage and handling of interference filters to ensure longevity.

Replacing the Bulb

Detailed steps for replacing the instrument's lamp and aligning it correctly.

Decontamination Procedure

Procedures for decontaminating the instrument, required before handling or shipping.

Chapter 6: Troubleshooting and Error Codes

Overview

Introduction to identifying and resolving potential error codes displayed by the ELx800.

Glossary of Terms

Definitions of common terms used in troubleshooting and error code interpretation.

Error Codes

Comprehensive list of error codes, their meanings, and probable causes.

General Errors

Details nonfatal error conditions requiring user attention and their solutions.

Fatal Errors

Lists critical errors requiring immediate attention and contact with technical support.

Appendix A: Computer Control

Overview

Introduction to controlling the ELx800 using Gen5, KC4, or KCjunior software.

Controlling the Reader With Gen5™

Setup and operation instructions for controlling the ELx800 with Gen5 software.

Controlling the Reader With KC4™

Setup and operation instructions for controlling the ELx800 with KC4 software.

Controlling the Reader With KCjunior™

Setup and operation instructions for controlling the ELx800 with KCjunior software.

Appendix B: Using 384-Well Geometry

Perform a 384-Well Plate Read in Gen5™

Guide for performing 384-well plate reads using Gen5 software with ELx800NB.

Perform a 384-Well Plate Read in KC4™

Guide for performing 384-well plate reads using KC4 software with ELx800NB.

Perform a 384-Well Plate Read in KCjunior™

Guide for performing 384-well plate reads using KCjunior software with ELx800NB.

Appendix C: Report Format

Appendix D: Comparison of the ELx800 and the EL800

Appendix E: Instructions for Programming a New Assay

Sample ANA Screen Enzyme Immunoassay Kit

Example of programming an assay with transformation and cutoff calculations.

Sample Human Anticardiolipin IgG Enzyme Immunoassay Kit

Example of programming an assay with standard curve and cutoff determination.

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