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Biotek Synergy H1 User Manual

Biotek Synergy H1
190 pages
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H
y
brid Multi-Mode Micro
p
late Reader
Synergy H1
TM
Operator’s Manual

Table of Contents

Other manuals for Biotek Synergy H1

Questions and Answers:

Biotek Synergy H1 Specifications

General IconGeneral
Detection ModesAbsorbance, Fluorescence, Luminescence
SoftwareGen5 Data Analysis Software
Light SourceXenon Flash Lamp
Wavelength Range200-999 nm
Plate Formats6- to 384-well plates
Monochromator Bandwidth9 nm
Temperature ControlYes
Automation CompatibilityYes
TypeMulti-Mode Microplate Reader

Summary

Chapter 1: Introduction

Product Description

Overview of the Synergy H1's features, modes, and capabilities.

Package Contents & Accessories

Lists all items included in the Synergy H1 package and their part numbers.

Optional Accessories

Details available accessories that can be purchased separately for the Synergy H1.

Materials for Conducting Liquid Tests

Lists necessary materials and part numbers for performing liquid-based tests.

Product Support & Service

Information on how to get help and support for the Synergy H1 product.

Technical Assistance Center (TAC)

Contact details and required information for technical support.

Applications Support

Information on how to get assistance with specific scientific applications.

Chapter 2: Installation

Product Registration

Instructions on how to register your BioTek product for important updates.

Important Information

Key notes and material requirements for the installation chapter.

Unpack and Inspect the Synergy H1

Step-by-step guide for safely unpacking and inspecting the instrument.

Install the Power Supply

Procedures for connecting the instrument's power supply.

Install the Dispense Module

Step-by-step instructions for installing the dispense module.

Connect the Host Computer

How to establish communication between the reader and the host computer.

Run a System Test

How to perform a system test to ensure proper operation.

Repackaging and Shipping Instructions

Procedures for safely packing and shipping the instrument.

Chapter 3: Getting Started

Modular Design

Explanation of the Synergy H1's modular design and upgrade options.

Filter Cube

Overview of the filter cube, its role, and types of filters/mirrors.

Gen5 Software

Introduction to using Gen5 software for instrument control and analysis.

Define the Filter Cube in Gen5 and on the Reader

Steps to configure the Gen5 optics library for installed filter cubes.

Dispense Module Control

Explanation of dispense functions (Prime, Purge) within Gen5.

Recommendations for Optimum Performance

Tips for achieving the best performance from the Synergy H1.

Chapter 4: Filters and Mirrors

Filter Cube Overview

Detailed description of the filter cube, its sets, and components.

Installing a Filter Cube

Step-by-step guide for installing a filter cube.

Change a Filter or Mirror

Detailed instructions on how to replace filters or mirrors in a filter cube.

Clean the Filters and Mirrors

Reference to cleaning instructions in Preventive Maintenance.

Chapter 5: Preventive Maintenance

Recommended Maintenance Schedule

Table outlining recommended PM tasks and frequencies.

Warnings and Precautions

Essential safety warnings and precautions for maintenance.

Clean Exposed Surfaces

Procedure for cleaning the exterior surfaces of the instrument.

Inspect/Clean Excitation and Emission Filters

Steps for inspecting and cleaning optical filters.

Flush/Purge Fluid Path

Routine for flushing and purging the fluid path for dispense models.

Clean the Dispense Tubes and Injectors

Routine cleaning of dispense tubes and injectors.

Chapter 6: As-Needed Maintenance

Decontamination

Procedures for decontaminating the instrument, especially before shipping.

Procedure for Models without the Dispense Module

Decontamination steps for models without the dispense module.

Procedure for Models with the Dispense Module

Decontamination steps for models with the dispense module.

Dispense Module, Syringe Replacement

Guide for replacing a faulty syringe in the dispense module.

Replace the Syringe

Step-by-step instructions for installing a new syringe.

Chapter 7: Instrument Qualification Process

Instrument System Test

Description of the automated system test performed on startup.

Absorbance Testing

Overview of absorbance qualification tests using plates and liquids.

Luminescence Testing

Overview of luminescence qualification tests using the Harta plate.

Fluorescence Testing

Overview of fluorescence qualification tests using plates and liquids.

Injection System Testing

Overview of tests to ensure injection system performance.

Chapter 8: Instrument Qualification Procedures

IQ/OQ/PQ Description

Explanation of Installation, Operational, and Performance Qualification.

Absorbance Plate Tests

Procedure for absorbance qualification using test plates.

Absorbance Liquid Tests

Procedures for absorbance qualification using liquid tests.

Luminescence Test

Procedure for luminescence qualification using the Harta plate.

Fluorescence Liquid Tests

Procedures for fluorescence qualification using liquid tests.

Injection System Tests

Procedures for testing the injection system.

Appendix A: Specifications

General Specifications

Overall specifications including microplate compatibility and environmental factors.

Absorbance Specifications

Detailed optical and performance specifications for absorbance mode.

Fluorescence Specifications

Detailed optical and performance specifications for fluorescence modes.

Luminescence Specifications

Performance specifications for luminescence detection.

Dispense/Read Specifications

Specifications related to the dispense module's accuracy and precision.

Appendix B: Error Codes

Overview

Explanation of how error codes appear in Gen5 and general troubleshooting.

Error Codes

Table listing common error codes and their descriptions/remedies.

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