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Biotek Synergy LX User Manual

Biotek Synergy LX
198 pages
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Synergy LX
Multi-Mode Microplate Reader
User Manual
ERRATA NOTICE: This document contains references to BioTek.
Please note that BioTek is now Agilent. For more information,
go to www.agilent.com/lifesciences/biotek.

Table of Contents

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Biotek Synergy LX Specifications

General IconGeneral
TypeMulti-Mode Microplate Reader
Wavelength Range (Absorbance)200-999 nm
Temperature ControlYes
ShakingYes
Automation CompatibilityYes
SoftwareGen5 Data Analysis Software
Detection ModesAbsorbance, Fluorescence, Luminescence
Excitation Wavelength Range (Fluorescence)250-900 nm
Plate Formats6- to 384-well plates
CO2/O2 ControlOptional
Emission Wavelength Range (Fluorescence)280-850 nm
Dynamic Range (Luminescence)>6 decades
Dimensions15.5" W x 16.5" D x 10" H (39.4 cm x 41.9 cm x 25.4 cm)
Weight35 lbs (15.9 kg)

Summary

Chapter 1: Introduction

Product Description

Describes the Synergy LX reader models, features, and supported plates.

Package Contents

Lists all items included in the Synergy LX instrument package.

Optional Accessories

Details available accessories that can be purchased for the Synergy LX.

Technical Support

Provides contact information and required details for technical assistance.

Chapter 2: Installation

Important Information

Crucial details regarding shipping hardware, packaging, and inspection.

Unpack the Box and See What’s Inside

Step-by-step instructions for unpacking the instrument and its accessories.

Remove Shipping Screw and Light-Blocking Plug

Procedure for removing shipping hardware and installing the light-blocking plug.

Prepare Host Computer (Gen5 Control)

Instructions for setting up the host computer for Gen5 software control.

Connect Host Computer and Reader

Steps for connecting the Synergy LX reader to a host computer.

Install the Power Supply

Instructions for connecting the instrument to a power source.

Turn on Reader and Run System Test

Procedure for powering on the instrument and running the initial system test.

Start Gen5 and Test Communication

Steps to start Gen5 software and verify communication with the reader.

Repackaging and Shipping Instructions

Guidelines for properly repackaging the instrument for shipment.

Chapter 3: Getting Started

External Components Overview

Identification of the instrument's external parts and ports.

Filter Cubes

Details on filter cubes for fluorescence/luminescence and their configuration.

Operate Reader Using Touchscreen

Guide to navigating and operating the Synergy LX via its touchscreen interface.

Configure Synergy LX

Setting essential instrument parameters like time, date, and formats.

Create and Save Protocols

Instructions for creating, saving, and managing assay protocols.

Run a Protocol

Procedure for selecting and running a protocol on the Synergy LX.

Recommendations for Optimum Performance

Tips and best practices for achieving optimal assay results.

Chapter 4: Periodic Maintenance

Maintenance Overview and Schedule

General maintenance guidelines and recommended schedules.

Warnings and Precautions

Critical safety warnings and precautions for performing maintenance.

Cleaning Procedures

Step-by-step instructions for cleaning instrument surfaces and touchscreen.

Filter and Bulb Maintenance

Procedures for inspecting, cleaning filters, and replacing the halogen bulb.

Decontamination Procedures

Guidelines for decontaminating the instrument, especially for biohazards.

Chapter 5: Instrument Qualification Procedures

IQ/OQ/PQ Description

Explanation of Installation, Operational, and Performance Qualification.

Recommended Qualification Schedule

Guidance on the recommended frequency for qualification tests.

System Test

Procedure for performing the instrument's automated system test.

Absorbance Testing

Methods and procedures for qualifying the absorbance system.

Absorbance Liquid Tests

Test procedures using liquid solutions to qualify absorbance performance.

Fluorescence Testing

Methods and procedures for qualifying the fluorescence system.

Luminescence Test

Procedure for qualifying the luminescence system using a Harta plate.

Appendix A: Specifications

General Specifications

Overall instrument specifications including dimensions, weight, and environment.

Absorbance Specifications

Detailed technical specifications for the absorbance measurement system.

Fluorescence and Luminescence Specs

Detailed technical specifications for optical measurements.

Appendix B: Sample Reports

Sample System Report

Example output of the instrument's system test report.

Sample Absorbance Test Plate Report

Example report for Absorbance Test Plate qualification.

Sample Assay Results

Example of raw assay data output in CSV format.

Appendix C: Error Conditions

Error Code Overview

Introduction to instrument error codes and basic troubleshooting.

Error Code Reference

A comprehensive list of error codes and their troubleshooting steps.

Appendix D: Safety Information

Safety Notices

General safety statements and multilingual notices.

Warnings and Precautions

Detailed warnings covering electrical, service, environmental, and chemical hazards.

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