EasyManua.ls Logo

Biotek ELx808 User Manual

Biotek ELx808
172 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
Page #1 background imageLoading...
Page #1 background image
Operator’s Manual
Absorbance Microplate Reader
ELx808

Table of Contents

Questions and Answers:

Biotek ELx808 Specifications

General IconGeneral
Wavelength SelectionFilter-based
Light SourceTungsten halogen lamp
DetectorSilicon Photodiode
TypeMicroplate Reader
Detection ModesAbsorbance
Plate Types96-well microplates
ShakingYes
InterfaceRS-232, USB
Filter Slots6-position filter wheel

Summary

Notices

Trademarks

Lists registered trademarks and company names.

Restrictions and Liabilities

Outlines limitations and responsibilities of the manufacturer.

Contact Information

Customer Service and Sales

Information on how to contact customer service and sales departments.

Service/TAC

Contact details for technical assistance and service.

Revision History

Document Conventions

Intended Use Statement

Specifies the designated purpose and limitations of the instrument.

Quality Control

Guidelines for ensuring accurate and reliable test results.

Warranty & Product Registration

Warnings

Important safety warnings regarding instrument operation and environment.

Hazards

Precautions

CE Mark

Directive 2004/108/EC: Electromagnetic Compatibility

Details compliance with EMC directives for emissions and immunity.

Directive 2006/95/EC Low Voltage (Safety)

Outlines compliance with low voltage safety directives.

Electromagnetic Interference and Susceptibility

USA FCC CLASS A

Specifies compliance with FCC Class A limits for digital devices.

User Safety

Safety Symbols

Chapter 1: Introduction

ELx808 Absorbance Microplate Reader

Describes the ELx808 as an eight-channel reader-assay system.

Reader Variations

Differentiates between standard and incubator/UV models.

Hardware Features

Lists the physical components and capabilities of the reader.

Software Features

Details the functionalities and capabilities of the onboard software.

Package Contents

Lists the items included with the reader.

Optional Accessories

Lists additional items available for purchase.

Specifications

Provides technical details like wavelength, filters, and measurement range.

Product Support and Service

Information on obtaining support and servicing for the instrument.

Chapter 2: Installation

Product Registration

Instructions for registering the product with the manufacturer.

1: Unpack and Inspect the Instrument

Procedures for unboxing and checking the instrument for damage.

2: Select the Operating Environment

Guidance on choosing a suitable location for instrument setup.

3: Install the Filter Wheel

Steps for installing the filter wheel into the reader.

4: Check/Adjust the Power Input Voltage Setting

Instructions for setting the correct input voltage for the instrument.

5: Connect Power

Steps for connecting the instrument to a power source.

6: (Optional) Connect Printer

Instructions for connecting an optional printer to the reader.

Printers

Information on compatible printers and connection via parallel port.

7: Turn on Reader and Run System Test

Procedure for powering on the instrument and performing a system self-test.

8: Check/Adjust the Reader’s Filter Table

How to ensure the software filter table matches physical filter locations.

9: Configure Reader Settings

Options for setting date, time, output, and read preferences.

SETUP Options

Steps to set the date and time on the reader.

OUTPUT Options

How to select report output devices and formats.

REPORT Type

Options for choosing the format of printed reports.

READ Options

Configuration of run-time prompts for plate and sample identification.

Establish Communication

Steps to set up communication between the reader and Gen5 software.

10: Install Software/Connect to Computer (Optional)

Guide for connecting the reader to a computer and installing software.

11: Verify Performance

Steps to verify instrument operation before initial use.

Before Repackaging the Instrument

Procedures for preparing the instrument for shipping.

Chapter 3: Operation

Getting Started with Gen5

Guide to creating and running experiments using Gen5 software.

Introduction

Overview of the reader's front panel interface and connectivity.

The Keypad

Explanation of the function of each key on the reader's keypad.

The Startup Screen

Describes the initial screens displayed during instrument power-up.

The Main Menu Screen

Overview of the main menu options and navigation.

Quick Read

A simplified method for quick assay programming and execution.

Defining Assays

Steps for customizing and setting up assay protocols.

Selecting an Assay

How to choose and load a predefined assay.

Assay Name

Procedure for naming or renaming an assay.

Defining the Method, Map, Formula, and Curve

Configuration of assay parameters like method, mapping, formulas, and curve fits.

METHOD

Defines the components of a reading method, including modes and parameters.

Read Type

Selection of assay read modes: Endpoint, Kinetic, or Scan.

Delay in First Read

Setting a time delay before the first measurement is taken.

Incubation Temperature

Setting the desired incubation temperature for assays.

Single or Dual Wavelength

Option to select single or dual wavelength readings for assays.

MEAS Selection

Selecting the appropriate filters for the assay measurement.

Number of Kinetic Reads/Kinetic Duration Selection

Setting the total number of reads or duration for kinetic assays.

Kinetic Interval

Defining the time interval between consecutive kinetic reads.

Kinetic Number of Reads

Specifying the total number of kinetic measurements to be performed.

Kinetic Duration

Setting the total time period for a kinetic reaction measurement.

Shake Mode Selection

Configuring the shaking parameters for the microplate.

Shake Time

Setting the duration for shaking the microplate.

Shake Speed

Selecting the speed for microplate shaking.

Kinetic Data Analysis Selection

Choosing the method for analyzing kinetic data.

Number of Kinetic Points Selection

Selecting the number of data points for kinetic analysis.

Onset OD Selection

Defining the time at which a specific optical density is reached.

Linear Scanning

Setting up parameters for linear scanning across wells.

MAP

Defining how blanks, controls, standards, and samples are assigned to plate wells.

Map Generation

Method for assigning well locations, either automatic or manual.

Mapping Direction

Selecting the direction (column or row) for mapping wells.

Replication Direction

Defining the direction for placing replicate samples.

Start Mapping at Well Location

Setting the starting point for automatic well mapping.

Selecting a Blank Map

Choosing the blanking method to apply to the assay.

Blank Map Definitions

Explains different blanking methods like AIR, FULL, CONSTANT, ROW, COLUMN.

Constant Blank Value Entry

Entering a specific absorbance value for constant blanking.

Number of Blanks

Specifying how many blank wells are used in the assay.

Selecting a Blank Location

Defining the physical location of blank wells on the microplate.

Number of Standards

Setting the quantity of standards to be used in the assay.

Number of Standard Replicates

Defining how many replicates of each standard are used.

Average Standards

Option to average standard replicates for curve calculation.

Standard Concentration

Entering the concentration values for each standard.

Reuse of Standard Curves

Instructions for reusing previously established standard curves.

Number of Controls

Specifying the quantity of control samples used in the assay.

Number of Control Replicates

Defining the number of replicates for each control.

Location of Controls

Specifying the physical location of control wells on the microplate.

Number of Samples

Setting the total number of samples to be read.

Number of Sample Replicates

Defining the number of replicates for each sample.

Sample Location

Specifying the physical location of sample wells on the microplate.

FORMULA

Setting up formulas for data validation, transformation, and classification.

Validation Formula Examples

Provides examples of blank, control, and assay validation formulas.

Formula Type

Selecting formula types: Cutoff, Transformation, and Validation.

Validation Type Selection

Choosing the specific type of validation formula to enter.

Formula Entry

Detailed steps for entering formulas using the reader's interface.

Number of Required Controls/Blanks

Specifying the number of valid controls or blanks for validation.

Cutoff Formulas

Calculating cutoff values for classifying sample results.

Greyzone Entry

Defining a greyzone around the cutoff for equivocal results.

Positive/Negative Calls for Cutoff

Assigning calls (POS, NEG, EQUIV) based on cutoff and greyzone values.

Transformation Formulas

Changing absorbance data format for further processing.

Transformation Scope Variable

Defining the scope (samples or all wells) for transformation formulas.

CURVE

Screens for editing curve-fit types, outliers, axes, and extrapolation.

Curve Fit

Selecting the method for fitting a curve to standard data points.

Edit Standard Outliers

Excluding or including standards from curve-fit calculations.

Axis Selection

Choosing the type of X and Y axes for curve plotting.

Extrapolation of Unknowns

Deciding whether to extrapolate curve data for values outside the standard range.

Panel Assays

Grouping up to 8 assays to run sequentially on a single plate.

Reading a Microplate

The process of initiating and performing a microplate read.

Select Assay

Selecting the assay to be used for the plate read.

Run-Time Prompts

Handling prompts for sample information during a read.

Enter Number of Samples

Inputting the total number of samples for the assay.

Enter Plate ID

Assigning a unique identifier to the microplate.

Enter Sample ID

Entering unique identifiers for each sample.

Beginning the Plate Read

The final step before starting the actual plate reading process.

Printing Reports and Assay Lists

How to generate and print various types of reports and assay lists.

Result

Viewing and selecting results for specific plate IDs and assays.

Editing Standard Outliers

Modifying standard outliers to improve curve-fit accuracy.

Printing Results

Printing the calculated results of an assay.

Map

Printing the plate map showing well assignments for an assay.

Assay

Printing detailed settings for a specific assay.

List

Printing a list of all assays stored in the reader's memory.

Recommendations for Optimum Performance

Tips and best practices for achieving optimal reader performance.

Chapter 4: Instrument Qualification

Overview

Introduces IQ, OQ, and PQ procedures for the ELx808.

Recommended Qualification Schedule

Defines recommended intervals for qualifying instrument performance.

Test Descriptions

Details various tests used for instrument qualification.

System Test

Describes the automated self-test performed by the reader.

Incubator Self Test

Verifies the functionality and temperature control of the incubator zones.

Checksum Test

Verifies the integrity of the reader's basecode software.

Absorbance Plate Test

Tests mechanical alignment, accuracy, and repeatability using a special plate.

Empty Carrier Test

Checks read capabilities without a microplate.

Liquid Tests

Procedures for testing performance with liquid samples.

Perform the Tests

Steps for executing the qualification tests on the instrument.

Perform Liquid Test 1

Step-by-step guide for conducting Liquid Test 1.

Perform Liquid Test 2

Instructions for testing linearity, repeatability, and accuracy with liquid samples.

Perform the Linearity Test (Test A)

Procedure for executing the linearity test using prepared solutions.

Calculate Repeatability (Test B)

Steps to calculate and verify the reader's repeatability.

Channel-to-Channel Variation and Alignment (Test C)

Verifying consistency across different optical channels.

Perform Liquid Test 3 (“UV” Models Only)

Optional test for linearity at 340 nm for UV models.

Calculate Linearity

Steps to calculate and verify the linearity of the reader's measurements.

Chapter 5: Preventive Maintenance

Recommended Maintenance Schedule

Defines tasks and frequencies for routine instrument maintenance.

Warnings and Precautions

Safety warnings and precautions before performing maintenance.

Clean Exposed Surfaces

Instructions for cleaning the external surfaces of the instrument.

Inspect and Clean the Wavelength Filters

Procedures for inspecting and cleaning the reader's wavelength filters.

(Optional) Lubricate Robotic Components

Maintenance steps for robotic models, including component lubrication.

Robotic Motor Adjustment Procedure

Steps to adjust the robotic door motor after lubrication or reassembly.

Replace the Lamp and Clean the Contacts

Instructions for replacing the instrument's lamp and cleaning contacts.

Decontamination

Procedures for decontaminating the instrument before handling or shipping.

Decontamination Procedure

Step-by-step instructions for decontaminating the reader.

Chapter 6: Troubleshooting and Error Codes

Overview

Introduces error code classifications and their implications.

Diagnostics

Recommends performing a System Self-Test for diagnosis.

Terms Used in the Error Codes Tables

Defines terms relevant to understanding error code descriptions.

Error Codes (General)

Lists general error codes and their probable causes.

Error Codes (Fatal)

Lists critical fatal errors requiring immediate attention.

Appendix A: Sample Reports

Samples with Calls on Matrix Report

Example of a report showing sample results with classification calls.

Curve Fit Report

Example report illustrating the curve fitting results.

Samples with Calls on Column Report

Example report displaying sample results and calls by column.

Column Report without Samples

Example column report excluding sample-specific data.

Panel Report

Example report for assays run as part of a panel.

Assay Detail Report

Detailed report of an individual assay's settings and parameters.

Assay List

Example list of all programmed assays in the reader.

Appendix B: Instructions for Programming a New Assay

Sample ANA Screen Enzyme Immunoassay Kit

Example of programming an assay with transformations and cutoffs.

Sample Anticardiolipin IgG Enzyme Immunoassay Kit

Example of programming an assay with a standard curve and cutoffs.

Expected Values

Typical results and interpretation ranges for the ANA assay.

Programming the Assay on the ELx808

Step-by-step guide for programming the ANA assay on the reader.

Calculate Mean Optical Densities

Calculating mean OD for duplicate assay readings.

Plot Calibration Curve

Methods for plotting calibration curves, automatic or manual.

Treatment of Anomalous Points

How to handle or remove anomalous data points from curves.

Calculation of Autoantibody Levels

Determining autoantibody levels from calibration curves.

Assay Calibration

Details on assay calibration against reference standards.

Expected Values

Typical results and interpretation ranges for the Anticardiolipin assay.

Programming the Assay on the ELx808

Step-by-step guide for programming the Anticardiolipin assay on the reader.

Appendix C: Adjusting the Power Input Voltage Setting

Identifying the Reader’s Power Input Module

How to identify the type of power input module installed in the reader.

Newer-Style Module

Procedures for newer modules, including voltage and fuse adjustments.

Adjust the Power Input Voltage Setting

Steps to adjust the voltage selector card for the correct input voltage.

Reconfigure or Replace Fuses

Instructions for changing or replacing fuses in the power input module.

Older-Style Module

Procedures for older modules, including voltage and fuse adjustments.

Adjust the Power Input Voltage Setting

Steps to adjust the voltage selector card for the correct input voltage.

Reconfigure or Replace the Fuses

Instructions for changing or replacing fuses in the power input module.

Related product manuals