Biotek Synergy HT User Manual

iv | Preface
BioTek Instruments
Operational/Performance Qualification...............................................30
Repackaging and Shipping Instructions .............................................31
Chapter 3: Getting Started ................................................................35
Key Components............................................................................36
Power Switch, Carrier Eject Button, Microplate Carrier ....................36
Lamp Assembly and Filter Wheel Access .......................................37
Excitation and Emission Filter Wheels ...........................................38
Installing the Time-Resolved Fluorescence Cartridge ......................41
Configuring the System for Luminescence Measurements................42
The External Dispense Module.....................................................43
Gen5 Software...............................................................................46
Viewing/Updating the Filter and Wavelengths Tables ......................46
Creating Protocols and Experiments .............................................47
Controlling the Dispense Module..................................................50
KC4 Software ................................................................................52
Viewing/Updating the Filter and Wavelengths Tables ......................52
Creating Protocols.....................................................................53
Reading Plates ..........................................................................55
Controlling the Dispense Module..................................................56
Recommendations for Achieving Optimum Performance .......................59
Chapter 4: Instrument Qualification..................................................61
Overview ......................................................................................62
IQ/OQ/PQ .....................................................................................62
Recommended Qualification Schedule ...............................................64
System Test ..................................................................................65
Description...............................................................................65
Procedure................................................................................66
Absorbance Plate Test.....................................................................70
Description...............................................................................70
Test Plate Certificates ................................................................71
Setup: Gen5.............................................................................72
Procedure: Gen5 .......................................................................73
Setup: KC4 ..............................................................................74
Procedure: KC4.........................................................................74
Results & Troubleshooting Tips....................................................78
Absorbance Liquid Tests ..................................................................80
Stock Solution Formulation ..............................................................81
Liquid Test 1.............................................................................82
Liquid Test 2.............................................................................83
Liquid Test 3.............................................................................86
Fluorescence Tests .........................................................................89
Required Materials.....................................................................90
Test Solutions...........................................................................91

Biotek Synergy HT Specifications

General

Brand	Biotek
Model	Synergy HT
Category	Laboratory Equipment
Language	English