SECTION M
Basic EZScan 2 Operation
1. Note: The M500 should be powered on before starting the EZScan software. The recommended
computer for the EZScan software is a Pentium class computer or faster running Windows 98 or
above. Do not multi-task while collecting data on EZScan.
2. Turn on the M500 if it is not already on, and allow it to warm up for about 30 minutes.
3. Begin EZScan by clicking on Start, Programs, EZScan and double click the EZScan Icon. If you
get an error, verify the instrument is connected to comm1. If it isn't refer to setting up the serial
connection. (800 x 600 screen setting on desktop is recommended)
4. Expand the EZScan Window box to fill screen with the maximize box next to the close button
located in the top right comer of the tool bar.
5. All sample scans need a background run. To scan a sample or background, choose Instrument,
Scan and the "Scan Sample" box will appear. The default scan range is 4000 to 600 cm
-1
. (A
smaller range can be selected but the M500 still has to scan to that starting point.) The default file
name is BUCK500, please change the name to one you will recognize as your sample or
background scan. (The file name must be no more than 8 letters and/or numbers long.) The
extension (. SCN) is automatically added. You can add a description of the scan in the Comments
box.
6. Close the sample and background scans after they are finished being scanned. From the toolbar
choose Data then Ratio Background. The "Ratio Background Spectrum" box will open and you
should type in the Background File Name, the Sample File Name and then OK. (If you put in an
incorrect file name a message box will appear and you have to restart Ratio Background.) The
true % T spectrum will then open and be auto-scaled on the screen.
7. From this point many options are available.
a. Change to Absorbance if you are doing quantitative work (Under Data).
b. Turn on the Cursor if you need an exact % T or Absorbance number (Under View). The
cursor will show the value of the X-Y of where you are pointing. Zoom into the area to make
sure you have the cursor on the line.
c. Under View you can set the Ordinate and Abscissa scales. (This moves you out of Autoscale
mode.) The "Set Ordinate Range" box lets you manually put in the minimum and maximum
displayed numbers with the keypad. The "Set Abscissa Limits" box sets the left and right cm
-1
with the keypad also.
d. While in the Autoscale mode before setting any limits you can drag a diagonal box around
one section of the scan. You will then zoom into that area. If you right click it will bring up
an option box. One of its options is "Undo Zoom". This will bring you back to your starting
point.
e. If you set the "Ordinate Range" the zoom function will only make a left and right box.
8. Printing: Once you have the area on the screen you want to print, double left click. This will
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