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CBS V3000AB - SAFETY; General Safety Warnings; Hazards Associated with Liquid Nitrogen

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2
WARNING
SAFETY
Go to custombiogenics.com/technicalguides.html
for more information.
IMPORTANT: The following section on SAFETY should be read carefully and followed completely. Warnings
regarding the handling of liquid nitrogen are by no means a complete volume on the safe use of cryogenic liq-
uids. All personnel should have complete knowledge of the correct procedures, as well as the hazards of work-
ing with liquid nitrogen. Failure to do so could result in serious injury or death. Be sure to read and understand
the Liquid Nitrogen Safety Data Sheet provided by your Liquid Nitrogen Supplier.
To avoid risk of electric shock, this equipment must only be connected to a supply mains with protective
earth.
The lid could present a crush hazard. Open the lid entirely and carefully to prevent the lid from
improperly engaging and falling. Use both hands while lowering lid into the closed position.
To prevent a rolling hazard, ensure the casters are locked.
HAZARDS ASSOCIATED WITH LIQUID NITROGEN:
Asphyxiation
When liquid is released into a conned space it displaces oxygen from the air. This results in an
oxygen-decient atmosphere, particularly if vented into a closed space, and asphyxia can result. Entering an
oxygen decient room can cause unconsciousness without warning. Always check air quality upon entering a
room where cryogenic liquids are being used, and if possible, have an air respirator available.
Cold Burns
Due to its extremely low temperature, the cold vapor and gas that are produced when liquid nitrogen boils
can give rise to cold burns or frostbite upon contact with unprotected skin. Exposure may also produce
discomfort in breathing and can provoke an asthma attack in susceptible individuals. Never allow direct skin
contact with liquid nitrogen or serious frostbite will result. Personal Protective Equipment (PPE) must be used,
based on the principle that it should prevent liquid nitrogen from touching the skin and eyes. Appropriate PPE
includes:
• Cryo-gloves (elbow length and loose tting).
• Safety goggles/full face shield.
• Long sleeve lab coat.
• Cryo-apron.
• Long pants that come over the top of shoes.
• Fully enclosed footwear.

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