9. Troubleshooting and Maintenance
Quick Help
Most issues concerning accuracy or reproducibility are sample, technique or protocol related and may be
resolved by following the suggestions below:
• Clean sample surfaces prior to dispensing a sample onto the measurement surface.
• Do not over or under fill the counting chamber. Follow the Count button dispense volume
directions. Volume is dependent on the chamber height specified in the protocol.
• Ensure samples are monodispersions of cells in solution. Vortex before loading into the count
chamber.
• Ensure cells are in focus before tapping the Count button.
• Ensure all sample concentrations fall within concentration limits of the instrument for accurate
results. When measuring samples with very low or very high cell densities, it is best to use a
modified protocol indicating the expected concentration.
• Review the protocol settings used to make the sample count. Some parameters such as cell size
limits, fluorescence intensity thresholds and cell roundness values may need to be empirically
determined for the cell type and culture solution in question. For example, when measuring
adipocytes, the cell diameter limits may need to be increased to capture the appropriate cell
population.
• Ensure cells have stopped moving before tapping the Count button. The time for cells to settle
may vary by cell type and should be determined empirically.
• Avoid introducing air bubbles into the sample solution when mixing or dispensing samples/
• Refer to the manufacturer of cell dyes such as Acridine Orange, Propidium Iodine or FDA for
assay specific troubleshooting guidance.
Software Errors
Pop-up messages will provide guidance for software-based errors or invalid user actions. In most cases,
simply re-launching the app will allow the user to continue to make measurements. Update the software
using the Updater app if experiencing a reoccurring software error.
Contact DeNovix Customer Support at 302-442-6911 or info@denovix.com if additional help is required.
Please include the serial number of your instrument and all pertinent data when contacting Customer
Support by email. The serial number may be found on the bottom of the instrument.
Outside of the US, please contact your local distributor for assistance.
Cleaning
Between Measurements:
• Use only a dry, lint-free cloth to wipe top and bottom sample surfaces. Check the live preview to
verify the sample has been removed.
• Optional: For additional disinfection or cleaning if sample residue has dried on the surface: Lower
the arm and pipette 10 uL of 70% ethanol or 10% bleach into the sample chamber. Wait
approximately 10 seconds. Lift the arm and wipe both the top and bottom sample surfaces with a
dry, lint free wipe.
Full Instrument Cleaning.
• To clean the entire instrument, spray 70% ethanol or 10% bleach onto a paper towel first and wipe down
the surface of the instrument.
• Do not use a spray bottle to douse the entire instrument. Excessive liquid may damage internal
components.
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