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General| Type | Automated Cell Counter |
|---|---|
| Light Source | LED |
| Data Export | CSV, PDF |
| Accuracy | ±5% |
| Measurement Type | Cell Count, Viability, Fluorescence |
| Detection Method | Fluorescence and Brightfield Imaging |
| Sample Volume | 10 μL |
| Analysis Time | <10 seconds per sample |
| Display | Touchscreen |
| Connectivity | USB, Wi-Fi |
| Precision | CV <5% |
| Power Requirements | 100-240 VAC, 50/60 Hz |
Overview of DeNovix CellDrop counters, integrated processors, and high-resolution touchscreens.
Highlights of the CellDrop instrument, including no consumables, stand-alone operation, and live preview.
Details on Brightfield illumination and Fluorescence excitation/emission wavelengths.
Precautions for handling biological samples and reagents like Trypan Blue.
Information on Wi-Fi, Ethernet, HDMI, and USB ports for data transfer and peripherals.
Specifies temperature and humidity requirements for indoor instrument use.
Emphasizes using only the provided power supply to avoid impairing protection.
Step-by-step instructions for performing a basic cell count measurement.
Recommendations for accurate and reliable cell counting, including sample preparation.
Describes the main navigation bar and its icons for easy software interaction.
Explanation of various standard icons and buttons used throughout the software interface.
Details the three chamber heights and their suitability for different cell densities.
Information on the instrument's storage capacity and automatic data management.
How the screen backlight turns off after inactivity and how to reactivate it.
Explains when the live preview is suspended and how to resume it.
Overview of available optical channels for Brightfield and Fluorescence measurements.
Describes individual count applications like Brightfield, Trypan Blue, AO, PI, GFP, and Yeast.
Details the PI app for counting dead cells using red fluorescence.
Explains the AO/PI app for counting live/dead cells using dual fluorescence.
Describes the GFP app for counting transfected cells using green fluorescence.
Details the Yeast app for counting live/dead yeast cells.
Allows users to create custom counting methods using available channels.
How to enter sample name, description, and passage number for tracking.
How to select and adjust count settings (protocols) like diameter and roundness.
Explains channel selection and focus adjustments for image preview.
How to adjust exposure for Trypan Blue and fluorescence channels.
How to zoom and pan images using touch gestures for better viewing.
Small map showing the current view portion of the full image.
Toggles between full screen and standard view for previews and results.
Tool to define target cell counts and calculate necessary dilutions.
Initiates a measurement and displays the required sample volume.
Options for outlining counted cells (Enhanced or Circle).
Selection between DirectPipette (default) and Slide modes.
Details on adjustable chamber heights for wide dynamic range and cell densities.
Improves counting accuracy for small cells near the detection limit.
How to set the dilution factor for accurate cell density calculations.
Setting minimum and maximum diameters for object detection during counts.
Settings to identify cells based on morphology and roundness.
Settings to evaluate cell intensity against protocol criteria.
Adjusting protocol parameters to optimize results after initial counting.
Returning to the count tab to start a new measurement.
Using sliders to gate cells by size and exclude debris from analysis.
Gating cells by intensity in the GFP application.
Options to select different image overlays for viewing results.
Describes controls like View Selector, Outlines, Export, and Image Selector.
Icons for selecting/deselecting items and editing/deleting results.
Modifying sample information stored in the database.
Removing highlighted samples from all results lists.
Explains different result views: Single Sample, Results List, Image List, Graph.
Available graph types and how to select them for analysis.
Specific graph types available for cell counts and distributions.
Other data views like Cluster # and % Viability.
How to take and export screen captures of the instrument interface.
Options for exporting results, settings, and images in various formats.
Details on selecting data types (.csv, .pdf, .png) for export.
Available destinations for exported data (USB, Email, Network Printer).
Creating and managing user accounts for customized settings and data segregation.
Overview of the account app's interface and available options.
Basic functions managed by the Account app, such as adding accounts and managing passwords.
Information on system accounts and user-added accounts.
How standard user accounts can be added and their limitations.
How administrator accounts are created and managed.
How to manage passwords for user accounts.
Setting up multiple email addresses for accounts.
Consequences of deleting an account and reassignment of protocols.
Searching, filtering, and managing saved data.
How data is automatically saved and exported.
Permanently removing selected data from the instrument database.
Managing pre-paid counts using codes on a per-account basis.
Mention of a calculator app to be implemented in a future release.
Setting up and using the instrument's timers.
Accessing and changing instrument settings like system info, date/time, and network.
Viewing software, firmware, IP addresses, language, and hostname.
Manual or automatic date and time configuration options.
Information on available language options for the interface.
Finding WiFi and Ethernet MAC and IP addresses.
Enabling/disabling automatic archive and network folders.
Adjusting volume controls for media and timer alarms.
Displaying current storage statistics.
Turning WiFi on/off and connecting/disconnecting from networks.
Configuring Ethernet connection.
Controls for screen brightness and sleep settings.
Mention of network printing feature not yet implemented.
Setting up email for notifications or export.
Recommended POP3 Gmail setup for outgoing email.
How to update CellDrop software and firmware to the latest versions.
Locking the screen for cleaning to prevent accidental input.
Mention of diagnostics app not yet implemented.
Tips for resolving accuracy or reproducibility issues, often sample or technique related.
Guidance for handling software errors and ensuring software is updated.
Procedures for cleaning sample surfaces between measurements and full instrument cleaning.
Information on compatible solvents and exceptions like Hydrofluoric Acid.
Details on product warranty against manufacturing defects.
Statement regarding document accuracy, completeness, and liability.
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