• Ensure that all sample concentrations fall within concentration limits of the instrument for
accurate results. When measuring samples with very low or very high cell densities, it is best
to use an alternative chamber height setting and sample volume.
• Review the protocol settings used to make the sample count. Some parameters such as cell
size limits, fluorescence intensity thresholds and cell roundness values may need to be
empirically determined for the cell type and culture solution in question.
• Ensure that cells have stopped moving before tapping the Count button. The time for cells to
settle may vary by cell type and should be determined empirically.
• Avoid introducing air bubbles into the sample solution when mixing or dispensing samples/
• Refer to the manufacturer of cell dyes such as acridine orange, propidium iodine or FDA for
assay specific troubleshooting guidance.
Software Errors
Pop-up messages will provide guidance for software-based errors or invalid user actions. In most
cases, simply re-launching the app will allow the user to continue to make measurements. Update the
software using the Updater app if experiencing a reoccurring software error.
Contact DeNovix Customer Support at 302-442-6911 or info@denovix.com if additional help is
required. Please include the serial number of your instrument and all pertinent data when contacting
Customer Support by email. The serial number may be found on the bottom of the instrument.
Outside of the US, please contact your local distributor for assistance.
Cleaning
Between Measurements:
• Use only a dry, lint-free lab wipe to clean top and bottom sample surfaces. Check the live
preview to verify the sample has been removed.
• Optional: For additional disinfection or cleaning if sample residue has dried on the surface:
Lower the arm and pipette 15 uL of 70% ethanol or 10% bleach into the sample chamber. Wait
approximately 10 seconds. Lift the arm and wipe both the top and bottom sample surfaces
with a dry, lint free wipe.
Full Instrument Cleaning.
• To clean the entire instrument, spray 70% ethanol or 10% bleach onto a paper towel first and
wipe down the surface of the instrument.
• Warning! Do not use a spray bottle to douse the entire instrument. Excessive liquid may
damage internal components.
Solvent Compatibility
CellDrop Series instruments are compatible with most solvents typically used in life science
laboratories.
Exception: Do not use Hydrofluoric Acid (HF) as the fluoride ion will etch the surfaces of the
measurement surfaces.
To Next Page
Loading...