DeNovix CellDrop FL - Count Settings; Cell Outline; Sample Mode; Chamber Height

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7. Count Settings
Each Count app includes an applicable group of count settings saved within protocols. Count settings
may be adjusted prior to a count. A subset of the parameters can be also adjusted after a count to
optimize a result.
• Cell Outline
o Enhanced Outline - Draws an outline around the identified cell boundary (recommended).
o Circle Outline - Marks each counted cell with a circle. The outlined image will be rendered
more quickly.
• Sample Mode
o DirectPipette - This is the default mode and is used when samples are loaded directly into
the CellDrop sample chamber.
o Slide - Choose this option when applications require cells to be contained in a slide while
counting.
• Chamber Height: A unique feature CellDrop Cell Counters is an adjustable chamber height
that allows for a greater dynamic range than any slide-based counter on the market. The
default chamber height is 100 µm and is recommended for most counts. For very low density
samples, choose 400 µm. For very dense samples, select 50 µm. The table below includes
guidelines.
Gap Height
Required Volume
Minimum Density
(cells/mL)
Maximum Density
(cells/mL)
400 um
40 uL
7 x 10
2
1 x 10
5
100 um
10 uL
5 x 10
4
1 x 10
7
50 um
5 uL
1 x 10
7
2.5 x 10
7
• Small Cell Mode: Small Cell Mode can improve brightfield channel counts of small cells near
the 4 um limit of detection. The additional algorithm complexity increases the length of the
analysis.
• Dilution Factor: This value is applied to the cell count when calculating cells/mL to account
for any dilutions or reagents added to the sample. The Dilution Factor is the ratio of the total
assay volume to the volume of cell suspension in the assay. Enter the proper dilution factor to
calculate the correct cell density.
o Example Dilution Factor calculations:
§ For pure cell suspensions, the Protocol Dilution Factor = 1.
§ For a mixture of 5 uL of assay working solution to 5 uL of cell suspension, set
Dilution Factor = 2.
§ For a mixture of 8 uL of assay working solution to 2 uL of cell suspension, set
Dilution Factor = 5.
• Diameter (um): The Diameter settings specify the size range of objects to seek during a
count. The algorithm rejects and does not process objects below the minimum size or above
the maximum limits. The limits define the boundaries of the data set, graphs and histograms.
The total allowable range is 2-400 um.
o Minimum Diameter - This setting defines the diameter of the smallest objects to be
counted. The proper value ensures that cellular debris will not be counted. Increase this