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Gentier Real-Time PCR System
▶ Analysis Mode: user can check the analysis mode check box of Reference Dye and Baseline
Gain Calibration according to the experiment requirements, and set the corresponding
parameters in the Baseline list.
a. Baseline Gain Calibration: in general conditions, the application software select the
Baseline Gain Calibration analysis mode by default, which provides user with Automatic
Baseline and Manual Baseline two baseline setting methods.
User can click the Automatic Baseline option, the application software will automatically
set the baseline for amplification curve, and shown the Start Cycle and End Cycle of the
automatic baseline setting in the coloumns of the Baseline list.
User can also click the Manual Baseline option, select one well in the Baseline list, use
and keys to maunually set the Start Cycle and End Cycle of the baseline setting for this
sample; or choose multiple wells and set the Start Cycle and End Cycle for All Selected
Rows on top of the Baseline list.
User can click < Restore > on the top right corner of Baseline list to restore the Start Cycle
and End Cycle ofthe automatic baseline setting.
b. Reference Dye: If user had set the reference dye in the Sample Setting tab according to the
experiment requirements, user can check the analysis mode check box of Reference Dye e
to analysis experiment data.
▶ Analytical Method: the application software provides user with two Ct value analytical
methods: Auto Threshold, Manual Threshold and Normalization Method.
a. User can click Auto Threshold option, the application software will automatically set and
show the threshold value for all dyes in its colounm.
b. User can also click Manual Threshold option, select one fluorescein and use and
keys to set the manual threshold value in its colounm.
c. User can click < Restore > on the top right corner of threshold list to restore the Auto
Threshold settings.
d. User can click Normalization Method option and the application software will
automatically calculate the Ct value according to the normalization value of amplification
curve.
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