4 Operation
4.3 Setting up a run
32 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
Table 4-2. The parameters of a gradient program.
Choose phase number
Choose phase number with and confirm with set enter. For a new program the
default phase number is 1. If the first phase has been programmed and the question
“Last phase ?“ is answered by No (N), the default number is 2 and so on.
Setting voltage, current and power
The display will now flash for the set voltage limit (STEP mode) or voltage endpoint
(GRADIENT mode). Using the keys, select the voltage limit or voltage endpoint
desired for the run. Confirm with set enter.
Repeat the same procedure for limiting current and limiting power.
Programmable values for voltage are 35-3500 V; current, 1-400 mA; power, 1-200 W.
Setting breakpoint
Choose between automatic or manual break. For automatic break, choose breakpoint
unit in either hours (h), volthours (Vh) or milliamperehours (mAh). Select the correct unit
or, for manual break, choose ”OFF” with . Confirm with set enter.
Note that the breakpoint unit is valid for all phases within the program and you will only
get this question when programming the first phase, see “Other phases” bypass in
Figure 4-1.
If h, Vh or mAh is chosen, the display will flash for the break value for the actual phase.
Set the value with and confirm with set enter. Programmable values for time are
0:01-500 h, volthours, 1-500 000 Vh, milliamperehours, 1-25 000 mAh.
If OFF is chosen, you have to break the electrophoresis manually by pressing STOP and
only one phase can be entered. The program will go back to the start position and the
program number will flash.
Last Phase?
After programming the breakpoint you are asked if this is the last phase or not. Select
Yes (Y) or No (N) with and confirm with set enter.
Phase number Voltage (V) Current (mA) Power (W) Volthours (Vh)
1 500 1 5 0:01
1
1
The ramping from 0 to 500 V should be done as quickly as possible. The smallest possible
time that can be set is 1 minute and the smallest possible Vh that can be set is 1 Vh. Vh
was chosen for this program as breakpoint unit.
1
1
2 500 1 5 5 2500
3350015510000
43500159.533250
To Next Page
Loading...
