4: Sample Experiments
5. Block the light path to the spectrometer. Then, take a dark spectrum by clicking the Store Dark
Spectrum icon on the toolbar or by selecting Spectrum | Store Dark from the menu bar. This
command merely stores a dark spectrum in memory. You must select File | Save | Dark from the
menu to permanently save the spectrum to disk.
Note
If possible, do not turn off the light source when taking a dark spectrum. If you must turn
off your light source to store a dark spectrum, allow enough time for the lamp to warm up
again before continuing your experiment.
You must take a dark spectrum before measuring relative irradiance.
6. Position the fiber at the light source you wish to measure. Then, choose the Irradiance mode
icon on the toolbar or select Spectrum | Relative Irradiance Mode from the menu bar.
7. Click the Save icon on the toolbar or select File | Save | Processed from the menu bar to save the
spectrum to disk.
Note
If you change any sampling variable (integration time, averaging, smoothing, angle,
temperature, fiber size, etc.), you must store a new dark and reference spectrum.
Time Acquisition Experiments
OOIBase32 allows you to perform time acquisition experiments. Time acquisition experiments track
processes, perform kinetic analyses, and monitor spectral events all as a function of time. You can collect,
as a function of time, spectral data from up to six single wavelengths (designated as Channels A through
F) and up to two mathematical combinations of these wavelengths (designated as Combinations 1 and 2).
Additionally, you can acquire data in any mode (transmission, absorbance, etc.).
For more details about this and other OOIBase32 functions, refer to the
OOIBase32 Spectrometer
Operating Software Online Help System
.
►
Procedure
Follow the steps below to perform a time series experiment in OOIBase32:
1. Enter scope mode and store a reference spectra and dark spectra.
2. Choose the measurement mode (absorbance, transmission, etc.) and select Time Acquisition |
Configure | Configure Time Channels from the menu bar to access the Time Acquisition
Channel Configuration screen.
Proceed to the Configuring the Time Acquisition Configuration Screen
section below.
26 220-00000-000-02-0605
To Next Page
