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Heinz Walz
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PAM-2500
Heinz Walz PAM-2500 User Manual
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Portable
Chlorophyll Fluorometer
PAM-2500
Handbook of Operation
2.156/08.2008
1. (preliminary) Edition: August 2008
PAM_2500_03pp.doc
©
Heinz Walz GmbH, 2008
Heinz Walz GmbH
•
Eichenring 6
•
91090 Effeltrich
•
Germ
any
Phone +49-(0)9133/7765-0
•
Telefax +49-(0)9133/5395
E-mail info@walz.com
•
Internet www.walz.com
2
Table of Contents
Default Chapter
3
Table of Contents
3
1 Safety Instructions
9
General Safety Instructions
9
Special Safety Instructions
9
2 Introduction
11
Intention of this Handbook
12
3 Components and Setup
13
Basic System Components
13
Basic System Setup
13
Pamwin-3 Software Installation
15
USB Serial Port Setting
16
USB Serial Port
16
Wireless Bluetooth Communication
17
Accessories
18
Field Combo (Optional)
18
External Battery 000160101314
18
Field Combo
18
Touch-Screen Ultra-Mobile Personal Computer
18
Automatic Charger 0001X
19
Additional Components
20
Distance Clip 60° 2010-A
20
Special Fiberoptics 2010-F
20
Leaf-Clip Holder
22
Leaf-Clip Holder 2030-B (Optional)
22
Micro-Quantum Sensor
23
Dark Leaf Clip DLC-8 (Optional)
25
Micro Quantum/Temp.-Sensor 2060-M (Optional)
25
4 PAM-2500 Operation
27
Pamwin-3 Help
27
Field Screen
27
Monitoring Graphs
27
Monitoring Graphs
28
Program and Script Control
29
Alphanumerical Area
29
Light Control
31
Fluorescence Data
34
Fluorescence Ratio Parameters
35
PS II Yield
35
Additional Data
37
First Measurements Using the Field Screen
38
First Measurements
38
Pamwin-3 Advanced Level
40
General Settings
40
Menu Bar
40
Dark Leaf Clip
45
Default
45
Distance Clip
45
RISC Processor
46
Induction Curve Parameters
47
Measuring Light Frequency
47
Global Settings
48
Fo, Fo
49
PAR Sensor Control
49
Pulses, Short Term Illumination
50
Tab Bar
50
Clock Event
52
Mode
52
Light Curve
53
New Record
53
SP-Analysis Mode
54
Slow Kinetics Window
54
Display Control
55
Graph Icons
55
Graph Y, X
55
Save Ft
55
Slow Kinetics Control
56
X-Axis Scaling/Zoom in
56
Display Control
57
Light Curve
57
Light Curves
57
LC (Light Curve) Control
58
Light Curve Edit
59
Light Curve Fit
59
PAR Sensor
59
Light Intensities
60
Report
60
Report Data Management
60
Report Window
61
Record Header and Last Line
62
Record Columns
63
Fast Acquisition Mode
64
Fast Settings
64
Basic Settings
65
Menu Bar
66
Target Averages
66
Trigger Settings ( I ) and ( II )
66
Trigger Tool Icons
66
Trigger Pulse
68
Light & Pulses
71
Fast Kinetics
72
Start Fast Kinetics
72
Cursor Coordinates
73
File Names
73
Fast Kinetics
74
File Menu
74
Zero Time Shift
75
Polyphasic Fluorescence Rise
76
View Mode
76
Icon Bar
78
Pick Data
78
Sidebar
78
Script Files
78
Fluorescence Ratio Parameters
78
5 Definitions and Equations
81
Relative Fluorescence Yields
81
Measurements with Dark-Acclimated Samples
81
Measurements with Light-Exposed (Treated) Samples
81
Fluorescence Ratio Parameters
82
Constant Fraction of Fo Fluorescence (C/Fo)
86
Relative Electron Transfer Rate (ETR)
87
Rapid Light Curves
89
Some Papers on Rapid Light Curves
91
Literature Cited in Chapter 5
92
Parameters
93
Par
93
6 Some Reviews on Chlorophyll Fluorescence
95
7 Specifications
97
Basic System
97
General Design
97
Light Sources
98
Special Fiberoptics 2010-F
98
Multiple Turn-Over Flash
98
Single Turn-Over Flash
98
Distance Clip 60° 2010-A
99
Battery Charger MINI-PAM/L
99
External Voltage Supply Cable
99
System Control and Data Acquisition
99
Carrier Bag
100
Transport Box 2040-T
100
Minimum Computer Requirements
100
Accessories
101
Ultra-Mobile Personal Computer for Field Research
101
Computer Box
101
Graph
101
Ultra-Mobile Touchscreen Computer SAMSUNG Q1 Ultra
101
External Battery 000160101314
102
Automatic Charger 000190101099
102
Leaf-Clip Holder 2030-B
102
Arabidopsis Leaf Clip 2060-B
102
Dark Leaf Clip DLC-8
103
Micro Quantum/Temperature-Sensor 2060-M
103
Suspension Cuvette KS-2500
103
Magnetic Stirrer MKS-2500
104
Compact Tripod ST-2101A
104
8 Trouble Shooting
105
9 Warranty
107
Conditions
107
Instructions
108
10 Index
109
4
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Heinz Walz PAM-2500 Specifications
General
Brand
Heinz Walz
Model
PAM-2500
Category
Measuring Instruments
Language
English
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