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IMAGING-PAM
Heinz Walz IMAGING-PAM User Manual
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Printed in Germany
IMAGING-PAM
M-series
Chlorophyll Fluorometer
Instrument Description
and
Information for Users
2.152 / 07.06
5. revised Edition: March 2014
imag-m-series0e_3.doc
Heinz Walz GmbH, 2014
Heinz Walz GmbH
Eichenring 6
91090 Effeltrich
Germ
any
Phone +49-(0)9133/7765-0
Telefax +49-(0)9133/5395
E-mail info@walz.com
Internet www.walz.com
2
Table of Contents
Default Chapter
3
Table of Contents
3
1 Safety Instructions
9
General Safety Instructions
9
Special Safety Instructions
10
2 Introduction
11
3 Components of the IMAGING-PAM MAXI-Version
15
Control Unit IMAG-CG
17
LED-Array Illumination Unit IMAG-MAX/L and IMAG- MAX/LR
19
CCD Camera IMAG-K6 and Objective K6-MAX
23
CCD Camera IMAG-K7 and Objectives K7-MAX/Z and K7- MAX/S
25
Mounting Stand with Eye Protection IMAG-MAX/GS
27
Leaf Distance Holder IMAG-MAX/B
32
Notebook PC IMAG-PC
33
Adapter IMAG-MAX/GWK
34
4 Components of the IMAGING-PAM MINI-Version
35
Multi Control Unit IMAG-CG
35
MINI-Head LED-Array IMAG-MIN/B and IMAG-MIN/R
36
CCD Camera IMAG-K6 or IMAG-K7
38
IMAG-MIN/GFP with IMAG-K6
40
Leaf Holder IMAG-MIN/BK with Grip Holder
46
Adapter for GFS-3000 (IMAG-MIN/GFS)
50
Imagingwin Software Versions for Various Types of MINI- Version
52
5 Components of the IMAGING-PAM MICROSCOPY-Version
54
Multi Control Unit IMAG-CG
55
CCD Camera IMAG-K6
55
Axio Scopea.1 Epifluorescence Microscope
57
Reflector Modules
59
Assembling of Beam Splitter and Filters
60
Mounting of the Reflector Module
62
LED Modules
64
Adjustment of Brightness by Grey Filters
64
Integration of LED Modules into Axio Scope.a1
66
Connecting LED Modules with IMAG-CG
68
Switching LED Modules for Measurements
68
Imag-Rgb
68
6 How to Get Started
71
Connecting the Cables
71
Software Installation
72
Installation and Starting of Imagingwin
72
Installation of Camera Driver
74
First Steps and Examples of Routine Measurements
74
7 Imagingwin
89
8 IMAGINGWIN - System Operation
91
Definition of New Record
91
Fo, Fm
91
New Record
92
Measure
92
Functions Applying to the View-Mode
93
Light Controls
95
9 IMAGINGWIN - Register Cards
98
Image-Window
98
Different Types of Images
98
Current Fluorescence Yield, Ft
99
Dark Fluorescence Yield, Fo
99
Fluorescence Yield, F
100
Maximal Fluorescence Yield, Fm
100
Maximum Fluorescence Yield, Fm
101
Maximal PS II Quantum Yield, Fv/Fm
101
Effective PS II Quantum Yield, y(II)
102
Quantum Yield of Regulated Energy Dissipation, y(NPQ)95
103
Quantum Yield of Nonregulated Energy Dissipation, y(NO)
104
Absorptivity, Abs
105
A Pparent Rate of Photosynthesis, PS/50
107
NIR Light Remission, NIR
108
Nonphotochemical Quenching, NPQ/4
109
Red Light Remission, R
110
Coefficient of Nonphotochemical Quenching, Qn
111
Coefficient of Photochemical Quenching, Qp
112
Coefficient of Photochemical Quenching, Ql
113
Inhibition, Inh
114
Image Capture and Analysis
115
Measure Abs
115
Area of Interest, AOI
116
Select: Fluorescence or Live Video
118
Zoom
120
Cursor
121
Analysis
121
Kinetics Window
124
Light Curve Window
130
Report Window
136
Settings Window
140
Light Parameters
141
Gain and Damping
144
Absorptivity
145
Slow Induction Parameters
146
Image Correction
146
Image Transformation
149
Battery
149
Display Parameters
150
Go Speed
152
PS Limit
152
Inh. Ref. AOI
153
Yield Filter
153
Fm Factor
154
F Factor
157
Reset Default Settings, Open or Save User Settings
160
High Sens. Window
161
Special SP-Routine
162
Fo Averaging
164
Fv/Fm Contrast Enhancement by Background Suppression156
164
RGB-Fit Window
165
RGB Gain
168
Fit Correction
168
10 IMAGINGWIN - Menu Bar
171
File
171
Transfer Fofm
171
Using Skript Files - Load Script/Run Script
171
Exit
179
Options
180
Ruler
180
Scale
180
Info Icons
181
Mean over AOI
181
Define AOI-Array Geometry
183
Create AOI Array
184
Al-List
186
LED Currents / PAR Values
186
Light Calibration
188
Recalc
189
Transect
190
11 List of Key Commands
193
12 Technical Specifications
194
Components Used in All Versions
194
Control Unit IMAG-CG
194
Imag-K7
195
Imag-K6
195
Windows Software Imagingwin
195
Battery Charger 2120-N
196
Components Specifically Relating to Maxi-Version
197
LED-Array Illumination Unit IMAG-MAX/L
197
LED-Array Illumination Unit IMAG-MAX/LR
198
Optional Filter Plate IMAG-MAX/F (Only for IMAG-MAX/L!)
199
External 300 W Power Supply
199
K7-Max/Z
199
K7-Max/S
200
K6-Max
200
K6-MAX/M and K7-MAX/M
201
Mounting Stand with Eye Protection IMAG-MAX/GS
201
Imag-Max/B
202
St-101
202
Transport Box IMAG-MAX/T
202
Imag-Max/Gwk1
203
Components Specifically Relating to MINI-Version
203
Imag-Min/B
203
Imag-Min/R
204
Imag-Min/Gfp
204
K7-Min
205
K6-Min
205
K6-Min/Fs
205
K7-MIN/M and K6-MIN/M
206
Imag-S
206
Imag-Min/St
206
St-1010
207
Imag-Min/Bk
207
Imag-Min/Gfs
207
Imag-Axioscope
207
Imag-L470M
208
Imag-L625M
208
Imag-Rgb
208
Imag-Ax-Ref
209
13 Warranty
210
Conditions
210
Instructions to Obtain Warranty Service
211
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Heinz Walz IMAGING-PAM Specifications
General
Brand
Heinz Walz
Model
IMAGING-PAM
Category
Measuring Instruments
Language
English
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