CHAPTER 9 IMAGINGWIN - REGISTER CARDS
136
only. The Booster is not implemented for the MICROSCOPY-
versions.
9.5.2 Gain and Damping
The Gain determines the amplitude of the
fluorescence signal, Ft, at a given setting of
Measuring Light Intensity (see 9.5.1). Twenty
settings are available, with standard setting 2
for MAXI- and MINI-versions. In the case of
the MICROSCOPY-versions, when dealing
with relatively low signal amplitudes, the Gain
9 is set by default. The Gain should be set such that in the absence of
actinic illumination the fluorescence amplitude (Ft = Fo) is in the
range of 150 - 200 units.
When dealing with weakly fluorescent objects (like diluted algae
suspensions in black multiwell plates using the MAXI-version) or in
MICROSCOPY-applications a Special SP-Routine is provided,
which involves automated switching to a high setting of Meas. Light
Int. at lowered Gain-setting (see 9.6.1). In this way, the Signal/Noise
of Fm, Fm', Fv/Fm and Y(II) measurements can be considerably
enhanced.
The time response of fluorescence measurement can be slowed
down by Damping. The selected Damping-setting determines the
time resolution with which changes of the Ft-image can be viewed
and also the amount of noise visible in the recorded images. Five
settings (0 - 4) are available, with standard settings 2. It has to be
considered that time resolution not only depends on Damping, but on
Measuring Light frequency as well (see 9.5.1). Hence, in order to see
rapid changes in the Ft-image, e.g. while moving a sample, both low
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