CHAPTER 9 IMAGINGWIN - REGISTER CARDS
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9.7.1 RGB Gain
The pixel intensity values of the Red, Green and Blue images are
derived from the pixel intensities of the corresponding Ft images
multiplied by the Red, Green and Blue Gains.
Upon pressing the RGB Gain button a window
upons which shows the current values of Red,
Green and Blue Gains. While the Green Gain is
fixed to 1.000, the Red and Blue Gains can be modified either
manually or via a special Measure routine (see below). Upon
instrument delivery Red and Blue Gains are set to 1.000, as the Red
and Blue ML intensities are adjusted to give appropriate R/G and
B/G ratios for optimal fitting using the standard Axiostar Plus
Epifluorescence microscope with the Fluar 20x/0.75 objective. With
other lenses for optimal fitting the Gains have to be adjusted (see
below).
Defined R/G and B/G ratios are a prerequisite for proper fitting
(deconvolution of differently pigmented algae). Adjustment of RGB
Gain may serve for compensating variations in RGB intensities. Such
variations may occur with ageing of ML LEDs and/or Liquid Light
Guide, choice of different objective lenses etc.
Note
The RGB Gain values have an influence on the RGB images
measured for the purpose of RGB Fit.
9.7.2 Fit Correction
The fit conditions for deconvolution of the 4
major groups of algae are preprogrammed and
are not directly accessible to the user. The Fit
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