Hettich ROTINA 420 User Manual

Rev. 03 / 11.2022 Andreas Hettich GmbH & Co. KG AB4701EN_SA
ROTINA 420
ROTINA 420 R
EN
Operating Instructions.................................................... 4

Hettich ROTINA 420 Specifications

General

Max Speed	15, 000 RPM
Capacity	4 x 100 ml
Temperature Range	-20 °C to +40 °C
Power Supply	230 V, 50/60 Hz
Type	Benchtop Centrifuge
Timer Range	1 - 99 minutes
Noise Level	< 60 dB(A)

Summary

1 Use according to specification

2 Remaining risks

3 Technical specifications

4 Notes on safety

5 Symbol meanings

6 Delivery checklist

7 Transport and storage

7.1 Transport

Instructions for safely transporting the device and accessories.

7.2 Storage

Guidelines for storing the device and accessories properly.

8 Unpacking the centrifuge

9 Initial operation

10 Interface (only for centrifuge with interface)

11 Opening and closing the lid

11.1 Opening the lid

Procedure for opening the centrifuge lid.

11.2 Closing the lid

Procedure for closing the centrifuge lid.

12 Installation and removal of the rotor

13 Inserting and removing hangers into/from the rotor

14 Inserting and removing the adapters into/from the hangers

15 Loading the rotor

16 Closing biosafety systems

17 Control and display elements

17.1 Control knob

Description of the control knob's function for parameter adjustment.

17.2 Keys and setting options

Explanation of the various keys and their setting options.

18 Enter centrifugation parameters

18.1 Running time

How to set the running time for centrifugation.

18.2 Starting runtime timing

Configuring when the runtime measurement begins.

18.3 Speed (RPM)

Setting the rotational speed of the rotor in RPM.

18.4 Relative centrifugal force (RCF) and centrifuging radius (RAD)

Setting RCF and RAD parameters for centrifugation.

18.5 Start-up and run-down parameters

Configuring acceleration and deceleration profiles.

18.6 Temperature (only for centrifuge with refrigeration)

Setting the desired temperature for cooled centrifuges.

19 Programming

19.1 Inputting or changing programs

How to save, modify, or create new programs.

19.2 Calling up programs

Retrieving saved programs for use.

19.3 Write protection for programs

Activating/deactivating program modification locks.

19.4 Program linkage

Connecting multiple programs to run sequentially.

19.5 Automatic temporary storage

Automatic saving of last run data to program place 0.

20 Centrifugation

20.1 Centrifugation with preselected time

Performing centrifugation based on a set time.

20.2 Continuous operation

Running the centrifuge without a time limit.

20.3 Short-time centrifugation

Performing centrifugation for brief durations.

21 Change the settings during the centrifugation run

22 Integral RCF

22.1 Query integral RCF

How to view the calculated integral RCF value.

22.2 Activate or deactivate the integral RCF display

Enabling or disabling the integral RCF display feature.

23 Emergency Stop

24 Cycle counter

24.1 After starting the first centrifugation run, enter the maximum permitted number of rotor cycles or deactivate the cycle counter

Initial setup for the rotor cycle counter.

24.2 Reset cycle counter to "0" and enter the maximum permitted number of rotor cycles

Resetting the cycle counter and setting max cycles.

24.3 Activating or deactivating the cycle counter

Enabling or disabling the rotor cycle counter.

25 Activating/deactivating the "Dual time mode" function

26 Activating or deactivating start-up and run-down times

27 Acoustic signal

28 Displayed centrifugation data after switch-on.

29 Set temperature unit (only for centrifuge with refrigeration)

30 Backlighting of the display

31 Setting program locks

32 PIN (Personal Identification Number)

32.1 Setting or changing the PIN

Procedures for setting or modifying the PIN.

32.2 Procedure in the event that PIN is lost

Steps to recover access if the PIN is forgotten.

33 Centrifuge address

34 Query the operating hours, centrifugation runs and cycle counter

35 Query the system information

36 Immediate display of centrifugation data after switch-on.

37 Cooling (only in centrifuges with cooling)

37.1 Standby-cooling

How the centrifuge cools when idle.

37.2 Precooling of the rotor

Process for rapidly cooling the rotor before use.

37.3 Time-controlled cooling

Setting a delay before cooling begins.

37.4 Prevent cooling being switched on during run-out

Disabling cooling during the run-out phase.

37.5 Temperature monitoring

Monitoring sample temperature to protect sensitive contents.

38 Heating (only on centrifuges with heating/cooling option)

39 Relative centrifugal force (RCF)

40 Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm³

41 Rotor recognition

42 Emergency release

43 Maintenance and servicing

43.1 Centrifuge (housing, lid and centrifuging chamber)

Cleaning and care for the main centrifuge body.

43.2 Rotors and Attachments

Maintenance procedures for rotors and accessories.

43.3 Autoclaving

Guidelines for autoclaving compatible accessories.

43.4 Centrifuge containers

Handling instructions for centrifuge containers.

43.2.1 Cleaning and care

43.2.2 Disinfection

43.2.3 Removal of radioactive contaminants

43.2.4 Trunnions

43.2.5 Rotors and accessories with limited service lives

44 Faults

45 Switch on automatic circuit breaker

46 Returning Devices

47 Disposal

48 Anhang / Appendix

48.1 Rotoren und Zubehör / Rotors and accessories

Technical specifications for various rotors and accessories.