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jenway 6850 User Manual

jenway 6850
56 pages
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Operating Manual
685 030 REV B/06-14
6850
Spectrophotometer

Table of Contents

Questions and Answers:

jenway 6850 Specifications

General IconGeneral
Brandjenway
Model6850
CategoryMeasuring Instruments
LanguageEnglish

Summary

Safety Information

General Safety Precautions

Provides essential safety information for operating and maintaining the spectrophotometer.

Introduction to the 6850 Spectrophotometer

Instrument Description

Overview of the 6850 spectrophotometer's features and capabilities.

Instrument Specifications

Detailed technical specifications for the 6850 spectrophotometer.

Spectrophotometer Installation and Setup

Unpacking and Included Items

Steps for safely unpacking the spectrophotometer and verifying included items.

Initial Installation Procedures

Guidance on setting up the spectrophotometer, including environmental requirements.

Display Interface Overview

Explanation of the instrument's display screen and its functions.

Instrument Controls and Keypad

Description of the keypad layout and function of each key.

Rear Panel Connections

Identification and function of ports and switches on the rear panel.

Front Panel Layout

Identification of components on the front of the instrument.

Spectroscopy Theory and Best Practices

Theory of Spectroscopy Measurement

Explains the principles of UV-visible spectroscopy and the Beer-Lambert Law.

Nucleic Acid Determination Methods

Details on measuring DNA and RNA concentrations and purity.

Spectroscopy Measurement Process

Describes the optical layout and measurement process of the spectrophotometer.

Good Laboratory Practice Guidelines

Recommendations for accurate and reproducible spectrophotometry measurements.

Spectrophotometer Instrument Setup

Instrument Navigation and Menu Access

How to navigate through the instrument's menus and select options.

Main Menu Screen Overview

Overview of the main menu options and available measurement modes.

System Utility Menu Functions

Accessing setup, service functions, and instrument settings.

Photometric Measurements Guide

Photometrics Menu Screen Options

Interface for adjusting photometric measurement parameters.

Photometric Method Setup

Configuration of wavelength, units, mode, and concentration factors.

Photometric Calibration

Procedures for zero calibration and calibration using a standard.

Photometric Sample Measurement

Steps for performing absorbance, %T, and concentration measurements.

Quantitation Measurements and Calibration Curves

Quantitation Mode Screen Overview

Interface for setting up and performing quantitation measurements.

Quantitation Wavelength Selection

How to select single or multiple wavelengths for analysis.

Quantitation Method Setup

Configuring units, curve fitting, and calibration tables.

Creating a New Standard Curve

Process for generating a calibration curve from standard solutions.

Storing Standard Curves

Saving a created calibration curve to the instrument's memory.

Recalling Stored Standard Curves

Loading a previously saved calibration curve for use.

Performing Quantitation Sample Measurements

Measuring samples using an established calibration curve.

Storing Quantitation Results

Saving measurement results to the instrument's internal memory.

Recalling Stored Quantitation Results

Accessing previously saved quantitation results.

Spectrum Scan and Analysis

Spectrum Mode Screen Details

Overview of the spectrum measurement interface and options.

Spectrum Method Setup

Configuring scan parameters like wavelength range and step.

Selecting Spectrum Measurement Mode

Choosing between Absorbance and % Transmittance modes.

Performing Spectrum Sample Measurements

Steps for acquiring a spectrum scan of a sample.

Adjusting Spectrum Scan Range

Modifying the displayed wavelength and photometric ranges.

Spectrum Peak Search Function

Locating peaks and critical points within a spectrum scan.

Spectrum Smoothing Application

Applying algorithms to smooth noisy spectral data.

Storing Spectrum Scan Results

Saving spectral data to the instrument's memory.

Recalling Stored Spectrum Results

Loading previously saved spectrum scans.

Kinetic Measurements Guide

Kinetics Mode Screen Overview

Interface for setting up and performing kinetic measurements.

Kinetics Wavelength Selection

Choosing the wavelength for kinetic measurements.

Kinetics Method Setup

Configuring run time, delay time, and interval settings.

Performing Kinetics Sample Measurements

Steps for acquiring kinetic data over time.

Adjusting Kinetics Scan Range

Modifying the displayed time and photometric ranges.

Calculating I/U Concentration in Kinetics

Determining concentration based on photometric changes over time.

Kinetics Spectrum Smoothing

Applying smoothing to kinetic scan data.

Storing Kinetics Results

Saving kinetic measurement data to the instrument's memory.

Recalling Stored Kinetics Results

Accessing previously saved kinetic data.

DNA and Protein Analysis

DNA/Protein Menu Options

Navigation and settings for DNA/Protein analysis.

Adjusting DNA/Protein Measurement Mode

Selecting calculation methods for nucleic acid and protein concentration.

DNA/Protein Wavelength Selection

Setting wavelengths for purity ratio and concentration calculations.

Adjusting DNA/Protein Calculation Factors

Modifying factors used in concentration calculations.

Selecting DNA/Protein Unit of Measurement

Choosing units for concentration results.

Resetting DNA/Protein Mode Settings

Restoring default parameters for the DNA/Protein mode.

Performing DNA/Protein Measurements

Steps to conduct DNA and Protein concentration and purity analysis.

Storing DNA/Protein Methods and Results

Saving analysis methods and results to memory.

Recalling Stored DNA/Protein Results

Accessing previously saved DNA/Protein analysis data.

Multi-Wavelength Measurements

Multi-Wavelength Mode Options

Overview of the multi-wavelength measurement interface.

Multi-Wavelength Selection

Defining multiple wavelengths for simultaneous measurement.

Performing Multi-Wavelength Sample Measurement

Steps for measuring absorbance/transmittance at multiple wavelengths.

Storing Multi-Wavelength Methods and Results

Saving analysis methods and results for multi-wavelength scans.

Recalling Stored Multi-Wavelength Results

Accessing previously saved multi-wavelength data.

USB Memory Stick Data Management

Saving and Recalling Data via USB

Instructions for using a USB memory stick for data storage.

Spectrophotometer Accessories and Spare Parts

Optional Accessories List

Catalog of optional accessories available for the instrument.

Installing Passive and Active Accessories

Guidance on fitting passive and active accessories to the instrument.

Using the 8 Position Automatic Cell Changer

Operation of the automatic cell changer accessory.

Spare Parts Identification

Part codes and descriptions for common spare parts.

Spectrophotometer Maintenance and Service

Routine External Maintenance

Keeping the instrument clean and protected.

Lamp Replacement Procedure

Step-by-step guide for replacing tungsten and deuterium lamps.

Contacting Service Department

Information on how to obtain technical support and service.

Troubleshooting and Technical Support

Troubleshooting Common Issues

Solutions for common problems encountered during sample measurement.

Technical Support Contact Information

Details for contacting Jenway's technical support team.

Declaration of Conformity

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