AQ-00073-000, Rev. 7 1
Introduction
The Labsphere RSA-PE-20 is a diffuse reflectance and transmittance accessory designed for a
number of PerkinElmer UV-VIS spectrometers. The list of compatible spectrometers includes
the Lambda 25/35/45 series as well as the older models Lambda 2/12/14/20/40.
The RSA-PE-20 accessory essentially is an optical bench, including transfer optics and an inte-
grating sphere, that fits neatly into the sample compartment of the spectrometer instrument. An
illustration of the accessory is provided in Figure 1. Since the reference beam passes through the
accessory unimpeded, the RSA-PE-20 accommodates either single or double beam configura-
tion spectrometer. The accessory, however, is a single beam device. The accessory is ideal for
measuring diffuse reflectance or transmittance for scientific and industrial applications including
color, thermochromism, composition of opaque materials such as biological or geological speci-
mens. The geometric measurement capabilities of the accessory include 8
°
/hemispherical
reflectance, 0
°
/diffuse reflectance, 0
°
/hemispherical transmittance and 0
°/
diffuse transmittance.
Sample holders for the test and reference samples are supplied with the accessory.
This manual provides the installation and operating procedures for the RSA-PE-20. The opera-
tion of your spectrometer system with the integrating sphere accessory installed, however, essen-
tially is unchanged. Since the procedures may vary from one spectrometer to the next, you
should consult your PerkinElmer instruction manual when taking reflectance and transmittance
measurements.
Figure 1. The RSA-PE-20 fits into the sample compartment of
your PerkinElmer spectrometer.