Leica Aperio AT2 DX - User Manual

Questions and Answers

J
Joe SandovalAug 3, 2025
What to do if my Leica Aperio AT2 DX scanner fails to start?
- B
  Bryan McdonaldAug 3, 2025
  First, check that the power switch at the back of the unit is turned on. Next, ensure that all scanner doors, including the AutoLoader carousel door, are completely closed, as the scanner will not start unless all doors are shut. Finally, click the Aperio Service Manager icon in the Windows task bar. Verify that the entry for the Controller has a green dot and says 'Stop Controller Service.' If it shows a red dot and says 'Start Controller Service,' click the entry to restart it.
M
Mark VelasquezAug 7, 2025
How to troubleshoot issues when scanning a slide with my Leica Aperio AT2 DX?
- R
  Richard GalvanAug 8, 2025
  Here are a few things to check when you're having trouble scanning a slide: * If the slide tissue is extremely thick or thin, this could cause issues. * If the slide is extremely dark or faint, use a parameter set such as faint. * If the label is large or located inside the area of interest, remove the label or adjust the area of interest. * For pen marks on the coverslip, remove the marks or perform a manual scan. * If there is dust or debris on the coverslip or bottom surface, clean the slide. * If there are bubbles under the coverslip, perform a manual scan. * In case of excess adhesive around the edges of the coverslip, clean it or perform a manual scan.
S
Sherry MooreAug 13, 2025
Why is the entire image out of focus on my Leica Aperio AT2 DX Laboratory Equipment?
- J
  john77Aug 13, 2025
  Make sure the slide is held flat and isn't moving in the tray during scanning; tape it down if needed. Also, the optic surfaces, such as the objective, may have fingerprints. Please refer to 'Clean the Objective' on page 41 for instructions.
M
Michael CaseyAug 18, 2025
What to do if autofocus doesn't detect enough focus points on my Leica Laboratory Equipment?
- J
  jimmy52Aug 18, 2025
  If the tissue is too faint to scan, try using the faint parameter set. Alternatively, try performing a new presnap.
M
Mary GravesAug 23, 2025
Why are some stripes of my Leica Aperio AT2 DX Laboratory Equipment image poorly focused?
- R
  Russell MartinAug 23, 2025
  This issue may indicate that the slide is moving during scanning. Please verify that the slide tray is clean and ensure that the slide is sitting flat and not rocking.
G
Gloria SalasAug 28, 2025
What should I do if the Tissue Finder includes a non-tissue area during a scan on my Leica Aperio AT2 DX?
- B
  Benjamin McdanielAug 28, 2025
  Perform a new presnap using a very clean, blank slide, and then repeat the manual load.

Summary

Introduction

Instrument Warnings

Highlights critical warnings for safe operation, focusing on electrical hazards.

Preparing for Scanning

Slide Preparation Tips

Tissue Preparation

Staining

Coverslips

Label Issues

Scanning Slides

Starting the Console

Barcode Requirement

Explains the necessity of barcodes for slide identification and quality control.

Manually Entering a Barcode

Multiple Slide Scanning Using the AutoLoader

Overview of automated scanning of up to 400 slides unattended.

Replacing a Rack

Loading a Slide into the Slide Tray

Viewing Scan Status

Batch Scanning

Procedure for performing the fastest possible scans of multiple slides.

Fine-Tuning Batch Scanning

Method for optimizing scans by taking and reviewing snapshots before scanning.

Scan Batch Tasks

Manually Scanning a Slide

Using Snapshots

How to capture, view, and use snapshots for scan area and focus point setup.

Working with Focus Points

Guidance on automatic and manual focus point placement for optimal image quality.

Automatically Adding Focus Points

Calibration

Z-Stack Scanning

Creating 3D images by scanning slides at multiple focal depths.

Basic Z-Stack Settings

Advanced Z-Stack Settings

Saving Z-Stack Settings as a Slide Setting

Image Quality Review

Importance of verifying scanned image quality for diagnostic accuracy by operators.

Using the AutoLoader

Microscope Slide Types

Slide Preparation

Loading Slides into the AutoLoader

Step-by-step guide for placing slide racks into the AutoLoader carousel.

Inspecting the AutoLoader and Racks

Quality Control and Maintenance

Maintenance Schedule

Recommended tasks and frequency for maintaining scanner performance and image quality.

Restart the Scanner Controller Service

Perform a Presnap

Set Line Camera Gains

Clean the Objective

Clean the Scanner

Cleaning the Scanner Interior

Cleaning the AutoLoader

Cleaning the AutoLoader Racks

Recalibrate the AutoLoader Slide-in-Rack Sensor

Procedure to ensure precise slide positioning from the AutoLoader to the stage.

Change the Aperio Service Account Password and Update ApService

Changing the Aperio Service Account Password

Updating the Aperio Service Credentials

Troubleshooting

General Troubleshooting

Common issues and solutions for scanner startup and general operation problems.

AutoLoader Troubleshooting

Specific solutions for errors related to the AutoLoader carousel status lights.

Slide on Stage

How to resolve errors when a slide is already present on the scanner stage.

Stalled AutoLoader Carousel or Rack Lift

Steps to clear obstructions and restart the AutoLoader carousel or rack lift.

Unable to Load AutoLoader Carousel

Troubleshooting for carousel stalling or inability to load racks.

Magnification Changer Troubleshooting

Addressing issues with the magnification changer not engaging correctly for scanning.

Recovering from a Motion Fault

Procedures to recover from stage position errors and rehome the stage.

Aperio AT2 DX Safety Instructions

Heed All Warnings

Importance of following all safety warnings listed in the manual and on the device.

Follow All Instructions

Emphasizes adherence to all operating and usage instructions for safe operation.

Heat

Ventilation

Biosafety Procedures

Water and Moisture

Warnings against using the scanner near water or in wet conditions to prevent hazards.

Attachments

Power Sources

Guidance on using the correct power source and checking voltage compatibility.

Grounding and Polarization

Importance of using the polarized AC plug with its integral safety ground pin.

Power Cord Protection

Lightning

Power Overloading

Warning against overloading electrical outlets with the scanner and accessories.

Operating Environment

Object and Liquid Entry

Prevention of objects or liquids entering the scanner openings to avoid electric shock.

Accessories

Moving

Servicing

Instruction to refer all servicing and repairs to qualified service personnel.

Damage Requiring Service

Conditions under which the scanner requires professional servicing for repair.

Replacement Parts

Importance of using specified manufacturer replacement parts for safety and function.

Operational Check

Requesting operational checks by a technician after repairs are completed.

Cleaning

Guidance on using a damp cloth for cleaning, avoiding liquid or aerosol cleaners.

Leica Aperio AT2 DX Specifications

General

Type	Digital Pathology Scanner
Capacity	Up to 400 slides
Magnification	20x, 40x
Z-Stacking	Yes
Scanning Method	Line scanning
Power Requirements	100-240 V AC, 50/60 Hz
Resolution	0.25 μm/pixel at 40x
Camera Type	CCD camera
Image Format	SVS
Operating System	Windows
Connectivity	Gigabit Ethernet