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Leica DM2700 M User Manual

Leica DM2700 M
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Leica DM2700 M
Instructions · Bedienungsanleitung

Table of Contents

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Leica DM2700 M Specifications

General IconGeneral
BrandLeica
ModelDM2700 M
CategoryMicroscope
LanguageEnglish

Summary

Important Notes about this Manual

Text Symbols and Pictograms

Explains text symbols, pictograms, and their meanings used in the manual.

Intended Purpose of the Microscope

Safety Notes

General Safety Notes

Provides general safety instructions for operating the microscope and its accessories.

Electrical Safety

Details electrical safety precautions, supply voltage, power input, and fuse specifications.

Transport and Storage

Guidelines for safely transporting and storing the microscope, including weight and environmental conditions.

Handling Light Sources

Safety precautions when handling light sources, noting irradiation risks and heat.

Handling Immersion Oil

Advises on safe handling of immersion oil, including skin contact and safety data sheets.

Handling Acids and Bases

Emphasizes caution when using acids or other aggressive chemicals with the microscope.

Disposal

Instructions for disposing of the microscope and its components according to regulations.

Type Labels

Shows examples of the microscope's type labels with technical specifications.

Overview of the Instrument

Instrument Dimensions

Provides the physical dimensions of the Leica DM2700 M microscope in millimeters.

Unpacking the Microscope

Installation Location

Details requirements for the installation location, such as dust-free environment and temperature stability.

Transporting the Microscope

Describes how to disassemble and package components for safe transportation of the microscope.

Microscope Weight

States the weight of the fully equipped microscope, exceeding 18 kg.

Assembling the Microscope

Assembling the Stage

Instructions for installing the microscope stage and specimen holder.

Assembling the Coaxial Pinion

Guides on mounting the coaxial pinion to either side of the stage and securing it.

Installing the Condenser

Steps for inserting and securing the condenser into the microscope's condenser holder.

Installing the Incident Light Axis

Procedure for installing the incident light illuminator before mounting the tube.

Installing the Tube and Eyepieces

Details on mounting the tube and inserting the eyepieces into the observation tubes.

Mounting Objectives

Instructions for selecting and mounting objectives, ensuring correct rotation and thread type.

Illumination Setup

Details on connecting lamp housings, including LED lamp housing LH113.

Lamp Housing 107/2

Procedure for removing and replacing the halogen lamp in the 107/2 lamp housing.

Lamp Housing 106z

Safety precautions and procedures for working with xenon burners and gas discharge lamps in the 106z housing.

Incident Light Turret Disk Setup

Instructions for inserting filter and reflector cubes into the incident light turret disk.

Analyzer and Polarizer Installation

Steps for installing the analyzer and polarizer components onto the microscope stand.

Lambda Plate Compensator

Procedure for attaching the lambda plate compensator to the microscope base.

ICR/ICT Prisms

Information on factory-mounted condenser prisms and adjustment for first use.

Optional Accessories and Cameras

Details on connecting cameras via adapters and calculating magnification on the monitor.

Connecting to the Power Supply

Final step to connect the microscope and lamp housing to the power supply using the correct cable.

Startup Procedures

Switching On the Microscope

Instructions for switching on the microscope, with a caution for gas discharge lamps.

Köhler Illumination Setup

Procedure for setting up Köhler illumination for transmitted light bright field.

Köhler Illumination for Incident Light

Procedure for setting up Köhler illumination for incident light bright field, including aperture diaphragm adjustment.

Phase Contrast Ring Check

Procedure to check and re-center phase contrast light rings for objectives.

Condenser Prism Adjustment

Procedure to check and adjust condenser prisms, especially after transport.

Adjusting Light Sources (106z Housing)

Centering and adjusting light sources specifically for the 106z lamp housing.

Centering Hg 50W Mercury Lamp

Detailed steps for centering the Hg 50W mercury lamp using adjustment windows and knobs.

Centering Hg 100W and Xe 75W Lamps

Steps for centering Hg 100W and Xe 75W lamps, including cautions about overheating.

Microscope Operation

Switching On for Operation

How to switch on the microscope for operation, noting external supply units for gas discharge lamps.

Stage and Object Movement

Instructions for lengthening the coaxial pinion and adjusting stage torque for X and Y movement.

Focusing the Microscope

Details on coarse and fine focusing wheels, their placement, and height adjustment.

Setting the Focus Stop

How to set a focus stop by locking the knurled wheel on the right-hand focus knob.

Adjusting Focus Drive Torque

Procedure for adjusting the torque of the focus drive using the knurled wheel.

Adjusting Microscope Tubes

Guides on adjusting interpupillary distance and viewing angle for various tube types.

Photo Tube Beam Splitting

Explanation of beam splitting settings for EDT22, BDT25+, and HC L 2TU photo tubes.

Using the Eyepieces

Instructions for using eyepieces, including aperture protectors and correction for vision problems.

Changing and Using Objectives

Procedure for changing objectives, checking settings, and using immersion objectives.

Light Source Adjustment

Adjusting brightness for LH113 lamp housing and notes on other light sources.

Aperture Diaphragm Adjustment

Explains the function of the aperture diaphragm and how it affects resolution, contrast, and depth of field.

Color-Coded Condenser Settings

Matching color markings on the condenser with objective color rings for aperture diaphragm settings.

Color-Coded Aperture Diaphragm (Incident Light)

Setting the aperture diaphragm to match the objective's colored ring for incident light.

Field Diaphragm Adjustment (Transmitted Light)

Using the field diaphragm to protect the specimen and enhance contrast, requiring adjustment for magnification changes.

Contrast Methods

Incident Light Contrast Methods

Covers Bright Field (RL), Oblique Illumination (RL), Polarization (RL), Interference Contrast (RL), Dark Field (RL), and Fluorescence.

Bright Field (Incident Light)

Basic setup for bright field illumination using incident light, including component settings.

Oblique Illumination (Incident Light)

Procedure for activating and adjusting oblique illumination for enhanced contrast.

Polarization (Incident Light)

Detailed steps for setting up polarization contrast using polarizers and analyzers.

Interference Contrast ICR (Incident Light)

Procedure for setting up Interference Contrast (ICR) using objective prisms.

Dark Field (Incident Light)

Setting up dark field illumination using a darkfield objective and reflector.

Fluorescence Illumination

Steps for setting up fluorescence observation, including selecting filter cubes and aperture diaphragm.

Transmitted Light Contrast Methods

Covers various transmitted light methods like bright field, phase contrast, dark field, polarization, and oblique illumination.

Bright Field (Transmitted Light)

Procedure for setting up bright field illumination using transmitted light, including filters and Köhler illumination.

Phase Contrast (Transmitted Light)

Setting up phase contrast for transmitted light, including objective selection and aperture diaphragm.

Dark Field (Transmitted Light)

Steps for setting up dark field for transmitted light using appropriate condensers and light ring slides.

Oblique Illumination (Transmitted Light)

Procedures for achieving relief-like contrast using oblique illumination in transmitted light.

Polarization (Transmitted Light)

Instructions for setting up polarization contrast in transmitted light, including analyzer and polarizer use.

Differential Interference Contrast ICT

Detailed steps for setting up Differential Interference Contrast (DIC) using polarizers and prisms.

Measurements with the Microscope

Linear Measurements

Required tools and procedure for performing linear measurements using the microscope.

Thickness Measurements

Method for measuring object thickness using transmitted light, considering refractive indices.

Using the Object Marker

How to use the object marker to scribe circles on the specimen for marking objects.

Troubleshooting Guide

Troubleshooting Microscope Stand Issues

Solutions for the microscope not responding, checking voltage, connections, and fuses.

Troubleshooting Illumination Problems

Addresses issues like a dark image or uneven illumination, lamp checks, and centering.

Troubleshooting Brightfield

Solutions for specimen not coming into focus and uneven illumination in brightfield.

Troubleshooting Darkfield

Addresses issues with no definite darkfield contrast and uneven illumination.

Troubleshooting Phase Contrast

Solutions for no phase contrast, covering specimen thickness, refractive indices, and light rings.

Troubleshooting Polarization

Addresses issues with no polarization contrast, checking polarizer/analyzer alignment.

Troubleshooting Transmitted Light Interference Contrast

Solutions for no transmitted light interference contrast, related to specimen and refractive indices.

Troubleshooting Incident Light Interference Contrast

Addresses no incident light interference contrast, covering specimen, cover glass, and prisms.

Troubleshooting Fluorescence

Solutions for dark fluorescence images or weak fluorescence, including lamp checks.

Care and Maintenance of the Microscope

Using the Dust Cover

Instructions for using the dust cover to protect the microscope and accessories from dust.

Cleaning Microscope Components

Guidelines for cleaning coated parts, optical surfaces, and avoiding harmful thinners.

Handling Acids and Bases

Precautions when using acids and aggressive chemicals, avoiding contact with optics and mechanical parts.

Changing Fuses

Procedure for changing fuses, emphasizing correct fuse types and replacement warnings.

Essential Wear and Spare Parts

Retrofitting Components

Fitting the Filter Magazine

Instructions for removing the base plate and inserting filters into the filter magazine.

Equipping the Condenser Disk

Procedure for equipping condenser disks with light rings, compensators, or lenses.

Inserting DIC Condenser Prisms

Steps for inserting DIC prisms into condenser disks, ensuring correct orientation and labeling.

Index

EU Declaration of Conformity

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