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Leica DM4000M User Manual

Leica DM4000M
69 pages
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Leica DM4000 B
Leica DM4000 M
Leica DM5000 B
Operating Manual
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Leica DM4000M Specifications

General IconGeneral
BrandLeica
ModelDM4000M
CategoryMicroscope
LanguageEnglish

Summary

Important Notes about this Manual

Safety Notes

General Safety Notes

Discusses safety class 1 device and user responsibilities for safe operation.

Electrical Safety

Provides general specifications for electrical safety of the CTR5000 box and microscope.

Overview of the Instrument

Imaging Procedure

Compares imaging procedures (transmitted, incident light) for different microscope models.

Transmitted Light Axis

Details specifications for the transmitted light axis of the microscope models.

Incident Light Axis

Details specifications for the incident light axis of the microscope models.

Objective Nosepiece

Details specifications of the objective nosepiece for different microscope models.

X/Y Stage

Details specifications of the X/Y stage for different microscope models.

Tube

Details specifications of the tube for different microscope models.

Condenser

Details specifications of the condenser for different microscope models.

Magnification Changer

Details specifications of the magnification changer for different microscope models.

Control Panels

Lists features of the control panels for the microscope models.

Computer Interface

Specifies the computer interface used with the microscope.

Software Tools

Lists software tools available for the microscope.

Unpacking the Microscope

Installation Location

Specifies environmental conditions and precautions for the installation location.

Transport

Provides instructions for disassembling and packaging components for transport.

Assembling the Microscope

5.1 Stage

Instructions for assembling the mechanical object stage onto the microscope stand.

5.2 Condenser

Instructions for inserting and connecting the condenser into the condenser holder.

5.3 Tube and Eyepieces

Instructions for mounting the tube and inserting eyepieces onto the microscope.

5.4 Objectives

Instructions for mounting objectives onto the objective turret.

5.5 Light Sources for the Transmitted Light Axis

Instructions for installing the 107/2 lamp housing for the transmitted light axis.

5.6 Light Sources for the Incident Light Axis

Instructions for installing the 106 z lamp housing for the incident light axis.

5.7 Equipping the Incident Light filter turret

Instructions for inserting filter and reflector cubes into the incident light turret.

5.8 Polarizer and Analyzer

Instructions for installing transmitted light and incident light polarizers and analyzers.

5.9 DIC Prisms

Instructions for inserting objective prisms for DIC.

5.10 Optional Accessories

Describes optional accessories like Ergomodule, Mirror Housing, Booster Lens, Excitation Manager.

5.11 Connection to the Power Supply

Instructions for connecting the microscope stand to the power supply.

5.12 Connection to the CTR5000 Electronics Box

Instructions for connecting the microscope to the CTR5000 electronics box.

Startup

6.1 Functional Principle

Explains the basic functional principles of the microscope and its controls.

6.2 Switching on the Microscope

Provides step-by-step instructions for switching on the microscope.

6.3 The Display (Leica DM4000 B/DM4000 M)

Explains the microscope display and its indications after initialization.

6.5 Köhler Illumination

Explains Köhler illumination and pre-set values for diaphragms.

6.6 Checking Phase Contrast Rings

Provides steps to check and level phase contrast light rings.

6.7 Adjusting the Light Sources

Instructions for adjusting transmitted and incident light sources.

Operation

7.1 Switching on the Microscope

Steps for switching on the microscope, including gas discharge lamps.

7.2 Stages and Specimen Displacement

Instructions for lengthening the coaxial pinion and adjusting torque.

Rotating the Stage

Instructions for revolving the stage and its range.

7.3 Focusing

Explains the use of focus dials for coarse and fine adjustment.

7.4 Tubes

Instructions for adjusting viewing distance, angle, and eyepiece extension.

Beam Splitting in Photo Tubes

Explains beam splitting for different photo tube types.

7.5 Eyepieces

Instructions for fitting eyepieces, including those with inlaid reticles and vision correction.

7.6 Objectives

Instructions for rotating objectives into place and automatic recognition.

7.7 Magnification Changer

Explains the use of the coded magnification changer.

7.8 Light Sources

Details how to set brightness for transmitted, incident, and fluorescence light sources.

7.9 Aperture Diaphragm and Field Diaphragm

Explains how to change aperture and field diaphragm settings.

Imaging Procedure for Leica DM4000 B/DM5000 B

8.1.1 Bright Field

Steps for bright field imaging using transmitted light.

8.1.2 Phase Contrast

Steps for phase contrast imaging using transmitted light.

8.1.3 Dark Field

Steps for dark field imaging using transmitted light.

8.1.4 Polarization

Steps for polarization imaging using transmitted light.

8.1.5 Differential Interference Contrast (TL)

Steps for DIC imaging using transmitted light.

8.2 Fluorescence

Steps for fluorescence imaging, including intensity and filter cube selection.

Imaging procedure for Leica DM4000 M

9.1.1 Bright Field

Steps for bright field imaging using incident light.

9.1.2 Dark Field

Steps for dark field imaging using incident light.

9.1.3 Polarization

Steps for polarization imaging using incident light.

9.1.4 Interference Contrast

Steps for interference contrast imaging using incident light.

9.2.1 Bright Field

Steps for bright field imaging using transmitted light.

Trouble Shooting

Stand

Troubleshooting issues related to the microscope stand not responding.

Illumination

Troubleshooting issues with dark or uneven illumination.

Bright Field

Troubleshooting issues with focusing in bright field.

Dark Field

Troubleshooting issues with achieving definite DF contrast.

Phase Contrast

Troubleshooting issues with obtaining phase contrast.

Polarization

Troubleshooting issues with polarization contrast.

Fluorescence

Troubleshooting issues with dark or weak fluorescence.

Display

Troubleshooting issues with the display flashing or showing "FAIL!".

Care of the Microscope

11.1 Dust Cover

Instructions on using and handling the dust cover.

11.2 Cleaning

General cleaning instructions for the microscope, stage, and glass surfaces.

Cleaning Objectives

Specific instructions for cleaning microscope objectives.

Removing Immersion Oil

Procedures for removing immersion oil safely.

11.3 Handling Acids and Bases

Precautions for handling acids and bases near microscope parts.

Essential Wear and Spare Parts

Abbreviations and Pictograms

Index

EU Declaration of Conformity

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