Quick Guide
11
Instructions for Use -
How to complete the first scan
All main functions for image acquisition and processing are accessible directly through
command buttons on the right-hand side of the screen. Most functions can be activated
and completed with a three-button mouse. A function is started with a left mouse click and
confirm it with a right click. Shortcut keys are also available for many functions. A complete
summary of all command buttons, menu commands, mouse clicks and key shortcuts can
be found in the accompanying manuals (§).
a The microscope should be switched on, ready for use (with AxioImager microscopes
wait until boot sequence is finished) and connected to the PC when the Metafer soft-
ware is started. If the communication with the microscope fails (e.g. objective, reflector,
filter wheel or shutter change takes more than 10 seconds), an error message is dis-
played and the microscope can be switched on or all microscope related functions are
disabled. To enable them again, please exit Metafer, solve the problem (e.g. switch on
the microscope and wait a few seconds until it is ready) and start again.
a Wait until the boot sequence of the microscope is complete, before you click the corre-
sponding program icon on the computer desktop. The Metafer software checks the
communication with the all relevant hardware components (microscope, camera, mo-
torized stage and slide feeder, if present). Only if all checks are successful, all Metafer
can be used. Otherwise, functions requiring hardware access is disabled. See Trouble-
shooting.
a Without Neon:
s On the Windows desktop, click on the Ikaros program icon.
a With Neon:
s On the Windows desktop, click on the ‘Neon’ program icon
s Load a case in the ‘Cases’ view or setup a new on by clicking the ‘Create Case’ com-
mand button in the ‘Create Case’ view.
s Go to the ‘Scans’ view and click on the ‘Metafer’ icon in the sidebar on the right
side.
a With the ‘Mode’ command you can select the desired operational mode.
a You must activate the required slide positions with a left mouse click on the fields 1 … 8
at the bottom of the program screen.
a Click on ‘Setup’ to define the search parameter: the ‘Name’ of the slide, the ‘Classifier’,
the ‘Search Window’ and its ‘Size’ as well as the maximum object count (‘Max. Cnt.’) must
be completed. Confirm the information by clicking ‘OK’. The software will then return to
the main program window.
a Click on ‘Search’ and follow the instructions on screen.
a The search is carried out by the system without any user interaction. After the search is
completed, images of all detected objects are displayed in gallery and saved including
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