Quick Guide
13
Microscopes
Microscopes are not part of MetaSystems systems. The camera and microscope must be
connected by a C-mount adapter for 2/3“ CCD cameras (not included). Please contact your
microscope representative for details. MetaSystems software supports a number of mo-
torized microscopes. Suitable control cables are available from the microscope manufac-
turers (not included).
A detailed description of supported microscopes and system configuration can be found in
the accompanying manuals (§).
Köhler Illumination
It is common knowledge among microscopists that correctly adjusted microscope is es-
sential for good image quality and, hence, the basis for a successful analysis of micro-
scope slide. The Köhler illumination provides a homogeneously illuminated field of view,
reduced optical glare from the light source and an increase in optical resolution. The pre-
requisite for Köhler illumination is a vertically adjustable, centerable condenser with iris
diaphragm. It is recommended to check the illumination on a regular basis (according to
laboratory SOPs):
1. Move up condenser with the top lens swung in (if available).
2. Focus on specimen with a low power objective (e.g. 10x or 16x).
3. Close down the lamp field stop (diaphragm in the microscope stand) while viewing.
4. Lower condenser slightly until the diaphragm (lamp field stop) image is in focus.
5. Center diaphragm image using the condenser centering screws.
6. Open diaphragm to the edge of field of view, then fine focus and open it further to just
clear the field.
7. Adjust contrast using the condenser aperture diaphragm.
8. Remove eyepiece and check to see that 75% of visible aperture is filled with light.
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