a Slide frame (slide frame
adapter on systems with
slide-feeder) not firmly
seated in scanning stage.
a Slide not firmly seated in
the slide frame.
a Incorrect microscope setup.
a Search area too large com-
pared to cell drop or tissue
section areas.
a Artifacts like trapped air
bubbles, oil droplets on the
cover slip, etc. .
a Defect focus drive: focus
drive is skipping focusing
steps.
a Auto-focus range is limited
by software settings.
a Remove slide frame/slide
adapter and reinsert it cor-
rectly.
a Insert slide correctly.
a Check the microscope set-
ting and consult the micro-
scope manual.
a Reduce the search area so
that there are objects al-
lowing focusing for most of
the image fields.
a Avoid artifacts during sam-
ple preparation and re-
move these artifacts from
existing slides.
a Run a simple test: focus a
sample manually, move
the focus drive a few steps
down and then the same
number of steps up again
(check the Z-position on
the microscope’s display):
the sample should be in fo-
cus again. If the sample is
not in focus, quit Metafer,
switch the microscope off,
wait 10 seconds, switch it
on again, wait another 10
seconds and then restart
Metafer. If the problem
persists, please contact
MetaSystems.
a Increased focus range in
Metafer settings (§ see
Metafer manual).
To Next Page
Loading...