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Measurement Concentration Range ........................................................ 9-1

Unique Screen Features .......................................................................... 9-2

Baseline Type .......................................................................................... 9-2

10. Protein BCA.......................................................................................... 10-1

Sample Volume Requirements............................................................... 10-1

Pedestal Reconditioning......................................................................... 10-1

Measurement Concentration Range ...................................................... 10-1

BCA Kits, Protocols, and Sample Preparation ....................................... 10-1

Unique Screen Features ........................................................................ 10-1

Making BCA Measurements................................................................... 10-2

Standard Curve Features....................................................................... 10-3

Delete Standard Points .......................................................................... 10-3

Exiting the BCA Module ......................................................................... 10-4

11. Protein Lowry ....................................................................................... 11-1

Sample Volume Requirements............................................................... 11-1

Pedestal Reconditioning......................................................................... 11-1

Measurement Concentration Range ...................................................... 11-1

Modified Lowry Kits, Protocols, and Sample Preparation....................... 11-1

Unique Screen Features ........................................................................ 11-1

Making Lowry Measurements ................................................................ 11-2

Standard Curve Features....................................................................... 11-3

Delete Standard Points .......................................................................... 11-3

Exiting the Lowry Module ....................................................................... 11-4

12. Protein Bradford................................................................................... 12-1

Sample Volume Requirement ................................................................ 12-1

Pedestal Reconditioning......................................................................... 12-1

Measurement Concentration Range ...................................................... 12-1

Bradford Kits, Protocols, and Sample Preparation ................................. 12-1

Unique Screen Features ........................................................................ 12-2

Making Bradford Protein Measurements................................................ 12-2

Standard Curve Features....................................................................... 12-3

Delete Standard Points .......................................................................... 12-3

Exiting the Bradford Module................................................................... 12-4

13. Cell Cultures......................................................................................... 13-1

Sample Size Requirements.................................................................... 13-1

Cell Suspension Concentrations ............................................................ 13-1

Sample Homogeneity............................................................................. 13-2

Decontamination of Measurement Pedestals......................................... 13-2

14. Archived Data and Data Viewer .......................................................... 14-1

Archive File Creation.............................................................................. 14-1

Data Storage Hierarchy.......................................................................... 14-1

Data Viewer............................................................................................ 14-2

Archive File Converter............................................................................ 14-5

Opening Archived Data with Spreadsheet Programs ............................. 14-6

15. Calibration Check................................................................................. 15-1

Procedure............................................................................................... 15-1

16. Troubleshooting................................................................................... 16-1

Error USB2000....................................................................................... 16-1

Connection Error .................................................................................... 16-2

Signal Error ............................................................................................ 16-2

Saturated Detector ................................................................................. 16-3

Liquid Column Breakage........................................................................ 16-3

Other Software Error Messages............................................................. 16-5

Sample Accuracy and Reproducibility.................................................... 16-6

260/280 Ratio......................................................................................... 16-7

Unusual Spectrum.................................................................................. 16-7

Technical Service................................................................................... 16-8

17. Maintenance and Warranty.................................................................. 17-1

Cleaning................................................................................................. 17-1

Calibration.............................................................................................. 17-1

Warranty................................................................................................. 17-1

18. Appendices........................................................................................... 18-1

Instrument Specifications ....................................................................... 18-1

Blanking and Absorbance Calculations.................................................. 18-1

Brand	NanoDrop
Model	ND-1000
Category	Measuring Instruments
Language	English

NanoDrop ND-1000 User Manual

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NanoDrop ND-1000 Specifications