Measurement Concentration Range ........................................................ 9-1
Unique Screen Features .......................................................................... 9-2
Baseline Type .......................................................................................... 9-2
10. Protein BCA.......................................................................................... 10-1
Sample Volume Requirements............................................................... 10-1
Pedestal Reconditioning......................................................................... 10-1
Measurement Concentration Range ...................................................... 10-1
BCA Kits, Protocols, and Sample Preparation ....................................... 10-1
Unique Screen Features ........................................................................ 10-1
Making BCA Measurements................................................................... 10-2
Standard Curve Features....................................................................... 10-3
Delete Standard Points .......................................................................... 10-3
Exiting the BCA Module ......................................................................... 10-4
11. Protein Lowry ....................................................................................... 11-1
Sample Volume Requirements............................................................... 11-1
Pedestal Reconditioning......................................................................... 11-1
Measurement Concentration Range ...................................................... 11-1
Modified Lowry Kits, Protocols, and Sample Preparation....................... 11-1
Unique Screen Features ........................................................................ 11-1
Making Lowry Measurements ................................................................ 11-2
Standard Curve Features....................................................................... 11-3
Delete Standard Points .......................................................................... 11-3
Exiting the Lowry Module ....................................................................... 11-4
12. Protein Bradford................................................................................... 12-1
Sample Volume Requirement ................................................................ 12-1
Pedestal Reconditioning......................................................................... 12-1
Measurement Concentration Range ...................................................... 12-1
Bradford Kits, Protocols, and Sample Preparation ................................. 12-1
Unique Screen Features ........................................................................ 12-2
Making Bradford Protein Measurements................................................ 12-2
Standard Curve Features....................................................................... 12-3
Delete Standard Points .......................................................................... 12-3
Exiting the Bradford Module................................................................... 12-4
13. Cell Cultures......................................................................................... 13-1
Sample Size Requirements.................................................................... 13-1
Cell Suspension Concentrations ............................................................ 13-1
Sample Homogeneity............................................................................. 13-2
Decontamination of Measurement Pedestals......................................... 13-2
14. Archived Data and Data Viewer .......................................................... 14-1
Archive File Creation.............................................................................. 14-1
Data Storage Hierarchy.......................................................................... 14-1
Data Viewer............................................................................................ 14-2
Archive File Converter............................................................................ 14-5
Opening Archived Data with Spreadsheet Programs ............................. 14-6
15. Calibration Check................................................................................. 15-1
Procedure............................................................................................... 15-1
16. Troubleshooting................................................................................... 16-1
Error USB2000....................................................................................... 16-1
Connection Error .................................................................................... 16-2
Signal Error ............................................................................................ 16-2
Saturated Detector ................................................................................. 16-3
Liquid Column Breakage........................................................................ 16-3
Other Software Error Messages............................................................. 16-5
Sample Accuracy and Reproducibility.................................................... 16-6
260/280 Ratio......................................................................................... 16-7
Unusual Spectrum.................................................................................. 16-7
Technical Service................................................................................... 16-8
17. Maintenance and Warranty.................................................................. 17-1
Cleaning................................................................................................. 17-1
Calibration.............................................................................................. 17-1
Warranty................................................................................................. 17-1
18. Appendices........................................................................................... 18-1
Instrument Specifications ....................................................................... 18-1
Blanking and Absorbance Calculations.................................................. 18-1
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