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Nikon Epiphot 300 User Manual

Nikon Epiphot 300
80 pages
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Inverted Metallurgical Microscope
EPIPHOT 300
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Nikon Epiphot 300 Specifications

General IconGeneral
BrandNikon
ModelEpiphot 300
CategoryMicroscope
LanguageEnglish

Summary

I. NOMENCLATURE

Microscope Main Components

Identifies and labels the primary physical parts of the EPIPHOT 300 microscope.

Microscope Accessories and Connections

Details external accessories and connection ports for the microscope.

Control Box and Power Supply Unit Components

Illustrates and labels the parts of the control box and power supply unit.

Camera Adapters and Dark Boxes

Shows and names components of the FX-35DX and NFX-35 dark boxes for photomicrography.

II. ASSEMBLY

Installation Environment and Lamphouse Adapter

Guides on selecting an installation site and attaching the lamphouse adapter.

Lamp and Lamphouse Installation

Provides safety cautions and steps for installing the microscope lamp and lamphouse.

Revolving Nosepiece and Stage Assembly

Instructions for correctly mounting the revolving nosepiece and stage onto the microscope.

Eyepiece Tube, Eyeguards, and Rotation Clamp Rings

Guides on attaching the eyepiece tube, optional eyeguards, and rotation clamp rings.

Mirror Block and Objective Installation

Details the process of inserting mirror blocks and screwing objectives into the nosepiece.

Power Supply Unit Connection

Explains how to connect the power supply unit to the microscope base.

Control Box Connection

Provides instructions for connecting the control box to the microscope and power.

Dark Box Attachment Procedures

Guides on how to attach the FX-35DX or NFX-35 dark box to the microscope.

Analyzer, Polarizer, and Reticle Slider Insertion

Instructions for inserting the analyzer, polarizer, and reticle sliders into their respective ports.

Photomicrographic Attachment Assembly

Steps for assembling and mounting a photomicrographic attachment to the microscope.

TV Camera Attachment and Projection Lenses

Details on mounting TV cameras with various projection lenses and adapters.

Other Light Sources: 12V-50W Halogen Lamp

Guides on using the 12V-50W halogen lamp and its associated adapter.

Lamp and Lamphouse Installation (12V-50W)

Instructions for inserting the 12V-50W lamp into the lamphouse and attaching it.

Metal Halide Light Source Assembly and Use

Guides on the assembly and operation of a metal halide light source.

III. PREPARATION

Diopter Adjustment Procedure

Step-by-step guide for adjusting diopters for clear binocular viewing.

Interpupillary Distance Adjustment

How to adjust interpupillary distance for aligning binocular viewfields.

Centering the 12V-50W Halogen Lamp

Procedure to center the 12V-50W halogen lamp for optimal illumination.

Centering the Field Diaphragm

Steps to center the field diaphragm for precise illumination control.

Centering the Aperture Diaphragm

Procedure to center the aperture diaphragm relative to the objective's exit pupil.

IV. MICROSCOPIC PROCEDURE

Brightfield Microscopy Setup

Guides on setting up the microscope for brightfield observation.

Darkfield Microscopy Setup

Instructions for configuring the microscope for darkfield microscopy.

Differential Interference Contrast Microscopy Setup

Steps for setting up the microscope for DIC observation.

Polarization Microscopy Setup

Guides for performing simplified and sensitive brightfield polarization microscopy.

V. OPERATION OF EACH PART

Filter Functions and Usage

Explains the purpose and application of various microscope filters.

Aperture Diaphragm Adjustment for Image Quality

How to adjust the aperture diaphragm to optimize resolution, contrast, and depth of focus.

Field Diaphragm Adjustment for Illumination Control

How to adjust the field diaphragm for precise specimen illumination and contrast.

Polarizer Slider Operation

Instructions for inserting and manipulating the polarizer slider for polarization microscopy.

Analyzer Slider and Red Compensator Usage

Guides on using the analyzer slider and first order red compensator in microscopy.

Mirror Block Attachment and Selection

Details on how to attach and use different mirror blocks for illumination.

Optical Path Changeover Lever Functions

Explains how to use levers to route light to different optical paths.

Eyepiece Tube and Zoom Dial Operation

Covers the functionality of the eyepiece tube and how to operate the zoom dial.

Focusing Device Operation

Instructions on using coarse/fine focus knobs and torque adjustment for precise focusing.

Rectangular Mechanical Stage Features

Details on the mechanical stage's handle, movement, and available stage rings.

Grain Reticle for Measurement

How to insert and use the grain reticle for measuring specimen features.

Micrometer Reticle for Precise Measurement

Instructions for using the micrometer reticle with different objectives for accurate measurements.

Optical System Best Practices

Notes on using objectives, projection lenses, and maintaining optical cleanliness.

VI. PHOTOMICROGRAPHY

Film Loading and Unloading Procedures

Guides for loading and unloading film for large format and 35mm photomicrography.

Exposure Mode Settings and Displays

Details automatic exposure modes, ISO settings, and shutter time displays.

Automatic Exposure Mode Photomicrography Guide

Step-by-step instructions for performing photomicrography using automatic exposure.

Magnification and Frame Composition for Photos

How to set magnification and compose frames for photomicrographic images.

Focusing Techniques for Photomicrography

Guides on focusing methods for different objective magnifications during photography.

Diaphragm and Voltage Adjustment for Photos

Adjusting diaphragms and voltage for optimal photomicrographic results.

Exposure Control Keys and Film Advance

Using EXP/RST keys for exposure, and managing film advance.

VII. TROUBLESHOOTING TABLE

Troubleshooting: Optical Path and Image Clarity Issues

Addresses issues with viewfield, focus, and image clarity caused by optical components.

Troubleshooting: Image Quality and Brightness Problems

Solves problems related to partially focused images, shifts, and brightness levels.

Troubleshooting: Operational and Fatigue Issues

Resolves operational problems like parfocality, image movement, and eye fatigue.

Troubleshooting: Electrical and Lamp Issues

Diagnoses and fixes electrical problems, including lamp and power supply faults.

Troubleshooting: Photomicrography Image Defects

Solves image issues in photomicrography like sharpness, blur, unevenness, and contrast.

Troubleshooting: Film, Exposure, and Ghosting Issues

Addresses problems with film, exposure, ghosting, and resolution in photomicrography.

Troubleshooting: Film Advance and Vignetting

Solves issues related to film advancement, double exposure, and vignetting.

VIII. CARE AND MAINTENANCE

Lens Cleaning Procedures

Detailed instructions for safely cleaning microscope lenses to maintain optical quality.

Large Format Mirror Cleaning

Procedure for cleaning the large format mirror if dust affects photomicrographs.

Surface Cleaning and Microscope Custody

Guidelines for cleaning painted surfaces and proper storage of the microscope.

ELECTRICAL SPECIFICATIONS

EPIPHOT 300 Microscope Specifications

Key technical specifications for the EPIPHOT 300 microscope itself.

Power Supply Unit (PSM-1120) Specifications

Technical details for the 100-120V input power supply unit.

Power Supply Unit (PSM-2120) Specifications

Technical details for the 220-240V input power supply unit.

Control Box Specifications

Technical data, fuse rating, battery, and environmental operating conditions for the control box.

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