Nikon eclipse E200 User Manual

ECLIPSE E200
Instructions
M313E 04.2.CF.2

Nikon eclipse E200 Specifications

General

Type	Biological Microscope
Optical System	CFI60 Infinity Optical System
Objectives	4x, 10x, 40x, 100x (Oil)
Observation Method	Brightfield
Magnification	40x to 1000x
Focus	Coaxial coarse/fine focus knobs
Eyepieces	10x
Condenser	Abbe condenser
Power Supply	AC 100-240V
Eyepiece Tube	Binocular Tube, Inclination 30°

Summary

Safety Precautions

Warning / Caution Symbols Used in This Manual

Explains warning and caution symbols used in the manual for user safety.

Meaning of Symbols Used on the Equipment

Explains symbols attached to the equipment and their meanings.

Intended Use of the Equipment

Details the primary applications and purpose of the microscope.

Do Not Disassemble

Warns against disassembling the instrument, which can cause malfunctions or void warranty.

Check Input Voltage

Instructs users to verify input voltage to prevent damage or fire hazards.

Use Specified Lamp, Fuse, and Power Cord

Emphasizes using only specified components to avoid damage or fire.

Heat at the Light Source

Warns about the heat generated by the lamp and precautions for touching it.

Hazardous Sample Handling

Provides guidelines for handling hazardous samples and decontamination.

Power Off Before Assembly/Maintenance

Instructs to turn off power before assembly, lamp/fuse replacement, or cord handling.

Keep Free of Moisture and Foreign Matter

Advises to prevent short circuits by keeping the microscope dry and clean.

Stage Rack Caution

Warns about the protruding stage rack to avoid injury.

Microscope Disposal

Advises on proper disposal to avoid biohazard risks.

Notes on Handling the System

Installation

Provides guidelines for proper installation of the microscope.

Carrying the Microscope

Instructions on how to handle and carry the microscope safely.

Handling the Lamps

Details on how to handle microscope lamps, including replacement precautions.

Refocusing

Explains how to refocus the specimen, especially when changing objectives.

Focus Knobs

Instructions on the proper use of focus knobs to avoid damage to the mechanism.

Oil-Immersion Observation

Details the procedure for oil-immersion observation, including handling immersion oil.

Shipping Clamps

Information about shipping clamps used for securing the microscope.

Nomenclature of Each Part

Microscope Components

Lists and describes the various components of the microscope.

Switches and Controls

Main Controls and Indicators

Details the function of various switches, knobs, and indicators on the microscope.

Quick Microscopic Procedure

Procedure for Microscopes Without Field Diaphragm

Step-by-step guide for initial microscope setup and observation without a field diaphragm.

Procedure for Microscopes With Field Diaphragm

Step-by-step guide for initial microscope setup and observation with a field diaphragm.

Microscopy: Detailed Procedure

Lamp Illumination

Instructions on how to turn on and adjust the lamp brightness.

Interpupillary Distance Adjustment

Guide to adjusting interpupillary distance for optimal viewing.

Changing the Eye Level

Explains how to adjust the eye level for comfortable observation.

Align Diopter Ring with Base Line

Procedure for aligning the diopter ring on the eyepieces with the engraved base line.

Specimen Mounting

Steps for placing and securing a specimen slide on the microscope stage.

Focus with the 10x Objective

Detailed steps for focusing the image using the 10x objective.

Focusing Techniques

Describes correct methods for focusing the microscope image to avoid damage.

Eyepiece Diopter Adjustments

Steps to adjust eyepiece diopters for clear binocular vision.

Field Diaphragm Centering and Focusing

Guide to centering and adjusting the field diaphragm for optimal illumination.

Objective Selection

Instructions on how to select and mount objectives onto the revolving nosepiece.

Aperture Diaphragm Adjustment

Explains how to adjust the condenser aperture diaphragm for optimal image contrast.

Miscellaneous Operations

Oil-Immersion Observation

Detailed procedure for oil-immersion observation, including sample preparation.

Handling Immersion Oil

Guidelines for using and storing immersion oil safely.

Adjusting Coarse Focus Knob Torque

Instructions for adjusting the tension of the coarse focus knob.

Upper Limit Bolt

Guide to installing the upper limit bolt to prevent objective contact with the specimen.

Eye Level Raiser

Details on installing and using the optional eye level raiser.

Photomicrography and TV Microscopy

Information on using the microscope for photomicrography and TV microscopy.

ND Filter for the Objective

Explains the use of ND filters for objectives to improve observation.

Cord Hangers

Describes how to install and use optional cord hangers for cable management.

Assembly

Assembly of Standard Set

Steps for assembling the standard set of the microscope, including voltage check and power connection.

Removal of Shipping Clamps

Steps to remove shipping clamps for safe transportation protection.

Installing the Eyepiece Tube

Guide on how to properly install the eyepiece tube.

Connecting the Power Cord

Instructions for safely connecting the microscope to a power source.

Assembly of Additional Components

Instructions for installing additional components like condenser, objectives, and eyepieces.

Condenser Installation

Detailed procedure for installing or replacing the condenser.

Objective Installation

Instructions for installing or replacing microscope objectives.

Specimen Holder Installation

Guide to attaching or removing the specimen holder.

Eyepiece and Eyepiece Pointer Installation

Steps for installing eyepieces and the optional eyepiece pointer.

Other Accessories Installation

Guidance for installing other optional accessories like photomicrographic equipment.

Replacement of Consumable Materials

Procedures for replacing consumable parts such as the lamp and fuse.

Replacing the Lamp

Step-by-step instructions for safely replacing the microscope lamp.

Replacing the Fuse

Instructions for safely replacing the microscope fuse.

Optical Characteristics

Objective and Eyepiece Combinations

Table detailing combinations of objectives and eyepieces with optical specs.

Microscope Terminology

Defines key optical terms like magnification, N.A., resolving power, and working distance.

Troubleshooting Tables

Optical Troubleshooting

Addresses optical issues like viewfield problems, poor image quality, and color cast.

Viewfield Dirt or Dust

Addresses issues of dirt or dust in the viewfield and related causes.

Poor Image Quality

Troubleshooting for low resolution, low contrast, or high contrast image issues.

Image Dark on One Side

Diagnoses and solutions for images appearing dark on one side.

Image Shifts During Focus

Troubleshooting steps for when the image shifts during focusing.

Image Tinged Yellow

Addresses causes and solutions for images with a yellow tint.

Image Too Bright

Troubleshooting for images that are too bright.

Insufficient Brightness

Solutions for issues related to insufficient illumination or brightness.

Mechanical Troubleshooting

Troubleshoots mechanical issues such as focusing problems, objective contact, and image shifting.

Focusing Issues with High-Power Objectives

Troubleshooting steps when unable to focus with high-power objectives.

Objective Contact Issues

Addresses problems with objectives contacting the slide after changing magnification.

Focal Point Differences Between Objectives

Solutions for significant focal point shifts when changing objectives.

Specimen Image Jumping

Troubleshooting for specimen images that jump when the stage is moved.

Binocular Images Not Integrated

Solutions for problems where binocular images do not merge properly.

Excessive Eye Fatigue

Addresses causes and solutions for eye fatigue during microscope use.

Electrical Troubleshooting

Diagnoses and resolves electrical problems related to the lamp and power supply.

Lamp Not Lighting

Troubleshooting steps when the lamp fails to turn on.

Unstable Lamp Brightness

Addresses issues of flickering or unstable lamp brightness.

Sudden Lamp Failure

Troubleshooting for sudden lamp failure, including incorrect lamp or voltage.

Care and Maintenance

Cleaning the Lenses

Instructions for cleaning microscope lenses using brushes, gauze, and alcohol.

Cleaning the Microscope Body

Recommendations for cleaning the microscope body with a silicon cloth or detergent.

Disinfecting the Microscope

Guidelines for disinfecting the microscope, especially after handling hazardous samples.

When Not in Use

Instructions for proper storage and covering of the microscope when not in use.

Periodical Inspections

Recommendations for regular inspections and maintenance to preserve performance.

Technical Specifications

General Specifications

Details model name, dimensions, weight, optical system, and focusing mechanism.

Electrical Specifications

Covers lamp rating, input voltage, frequency, current, fuse, and power cord details.

Electrical Specifications for 220-240V Areas

Specific electrical details for models operating in 220, 230, 240 V regions.

Operating Environment

Specifies recommended room temperature, humidity, altitude, and pollution levels.

Storage and Transport Conditions

Details environmental conditions for storage and transport of the microscope.

Conforming Standards

Lists standards and directives the microscope models comply with.