Chapter 8 Troubleshooting Tables
1 Optical
78
Dirt or dust in the viewfield.
Causes Corrective Measures
Condenser position too low.
If the microscope is equipped with a
field diaphragm, correct positioning so
that the field diaphragm image is
focused on the specimen surface. (P.57)
If the microscope is not equipped with a
field diaphragm, position the condenser
slightly lower than the upper limit.
Aperture diaphragm closed too far.
Open properly. (P.59)
Dirt or dust on the lens (condenser,
objective, field lens, eyepiece,
specimen).
Clean the lens. (P.84)
Poor image quality (low resolution, contrast too low or too high)
Causes Corrective Measures
Condenser position too low.
If the microscope is equipped with a
field diaphragm, correct positioning so
that the fie ld diaphragm image is
focused on the specimen surface. (P.57)
If the microscope is not equipped with a
field diaphragm, position the condenser
slightly lower than the upper limit.
Cover glass too thick or thin.
Use a cover glass of the specified
thickness (0.17 mm).
Slide upside down.
Turn over the slide so that the cover
glass faces up.
No cover glass attached to the slide.
Attach a cover glass 0.17 mm thick.
No immersion oil used on the front lens
of the oil-immersion objective.
Apply Nikon immersion oil to the
objective. (P.62)
Nikon immersion oil is not used for
oil-immersion observation.
Use Nikon immersion oil. (P.62)
Air bubbles in immersion oil.
Remove bubbles. (P.62)
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