Chapter 4 Microscopy (Detailed Procedure)
58
(2) While looking into the eyepieces, rotate
the condenser focus knob to focus the
field diaphragm image on the specimen
surface. (Focus on the edge of the
diaphragm image.)
(3) Rotate the field diaphragm centering
screws until the field diaphragm image
comes to the center of the viewfield.
(4) Switch to the 40x objective and rotate
the field diaphragm ring so that the field
diaphragm image becomes about the
same size as the viewfield.
(5) If the field diaphragm image is not
centered, use the centering screws
again.
8
Objective Selection
Rotate the revolving nosepiece to the desired objective magnification. (The objective will click
into place when rotated into position.)
Adjust the aperture diaphragm lever according to the selected objective. If the microscope is
equipped with a field diaphragm, also adjust the size field diaphragm (see the procedures 9 and
10).
9
Aperture Diaphragm Adjustment
Set the condenser aperture diaphragm lever to the
same figure as the magnification of the objective in
the optical path.
Focus on the
field
diaphragm
image with
this knob.
Field dia
hra
m ima
e
Then, center
the image.
O
en the field
dia
hra
m till
the field
diaphragm
image
circumscribes
the viewfield.
Focus.
Focus on the
field
diaphragm
image with
this knob.
Then, center
the image.
Corres
ondin
ob
ective ma
nification
A
erture dia
hra
m
lever
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