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Nikon Eclipse E400 User Manual

Nikon Eclipse E400
33 pages
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Instructions
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Nikon Eclipse E400 Specifications

General IconGeneral
TypeBiological Microscope
Optical SystemCFI60 Infinity Optical System
NosepieceQuadruple nosepiece
Magnification40x to 1000x
Power SupplyAC 100-240V, 50/60Hz
Lamp Power6V 30W
Eyepiece10x
Objective Lenses4x, 10x, 40x, 100x
StageMechanical stage
FocusCoaxial coarse and fine focusing
CondenserAbbe condenser, N.A. 1.25
Observation TubeBinocular
Stage Dimensions134mm
Interpupillary Distance Adjustment50-75mm
Diopter Adjustment+/- 5 diopters
IlluminationBuilt-in Koehler illumination

Summary

Before Using the Microscope

Warning and Caution Symbols

Explains warning and caution symbols used in the manual for safety.

Product-Specific Symbols

Details symbols found on the microscope itself and their meanings.

Microscope Safety and Environment

Installation Location Guidelines

Guidelines for selecting a suitable environment for microscope setup to ensure precision.

Lamp Handling and Replacement

Cautions regarding the hot lamp and safe replacement procedures, including cooling times.

Stage Operation Safety Precautions

Warnings about potential hand injuries from protruding stage parts during operation.

Additional Microscope Safety

Preventing Moisture and Liquid Damage

Instructions on handling spills and preventing water damage to the microscope.

Microscope Handling and Carrying Best Practices

Proper techniques for safely carrying and handling the delicate microscope.

Microscope Component Identification

Optimizing Image Quality

Condenser Aperture Diaphragm Adjustment

How to adjust the condenser aperture for optimal image contrast and definition.

Diopter Adjustment for Clear Binocular Vision

Steps for adjusting diopter for comfortable and clear binocular observation.

Image Enhancement Techniques

Condenser Focusing and Centering

Procedures for aligning the condenser for sharp, centered illumination.

Color Balancing Filter (NCB11) Usage

How to use the NCB11 filter for natural color tone in images.

Adjusting Lamp Voltage for Color Tone

Adjusting lamp voltage to influence the color tone of the illumination.

Initial Microscope Setup and Adjustment

Diopter and Interpupillary Distance Adjustments

Crucial adjustments for user comfort and image clarity before microscopy.

Specimen Placement on Stage

Magnification and Illumination Control

Condenser Aperture Diaphragm Adjustment

Detailed guide on adjusting the aperture diaphragm for different objectives.

Field Diaphragm Adjustment for Contrast

How to adjust the field diaphragm to limit illumination and improve contrast.

Photomicrography Setup

Eyepiece Tube Selection and Installation

Guidance on choosing and installing eyepiece tubes for photomicrography.

Minimizing Vibrations with Eyepiece Levers

Using the "NO CLICK" switch to minimize vibrations during photomicrography.

Photomicrography Adjustments

Selecting Color Tone and Brightness

Adjusting lamp voltage and using filters for optimal color and brightness.

Stage Trimming for Positioning

Adjusting stage rotation for precise specimen positioning during photography.

Focus Lock Mechanism

Coarse Focus Stopper Operation

How to use the coarse focus stopper for quick rough focusing and locking.

Adjusting Control Knob Torque

Coarse Focus Knob Torque Adjustment

Adjusting the resistance of the coarse focus knob for easier manipulation.

Stage Motion Control Knob Torque

Adjusting tension for X and Y axis stage motion control knobs.

Condenser Selection Guide

Immersion Oil and Water Operations

Oil-Immersion Operation Precautions

Procedures and precautions for using immersion oil with objectives and condensers.

Water-Immersion Operation Guidelines

Guidelines for using water-immersion objectives and handling water.

Microscope Assembly and Disassembly

Tools and Input Voltage Confirmation

Lists necessary tools and checks input voltage before assembly.

Safety During Microscope Assembly

Warnings and cautions to prevent electrical shock and damage during assembly.

Power Cord and Accessory Connections

Connecting the Power Cord Safely

Proper procedures for connecting the microscope's power cord.

Installing Separately Sold Accessories

Guidance on installing separately sold accessories according to their manuals.

Microscope Component Units Overview

Replacing the Microscope Lamp

Condenser Installation and Alignment

Condenser Installation Procedure

How to physically install the condenser onto the microscope.

Condenser Focusing and Centering Steps

Procedures for aligning the condenser for optimal illumination.

Stage and Objective Installation

Installing the Microscope Stage

Steps for mounting the stage onto the substage correctly.

Installing Microscope Objectives

How to screw objectives into the revolving nosepiece.

Eyepiece Tube and Riser Installation

Installing the Eyelevel Riser

How to attach the eyelevel riser for adjusting eyepiece height.

Installing the Eyepiece Tube

Procedures for attaching the eyepiece tube to the riser or arm.

Eyepiece and Lens Maintenance

Installing the Eyepieces

Steps for inserting the left and right eyepieces into the tube.

Lens Cleaning Procedures

Guidelines for cleaning various lenses using appropriate cleaning agents.

Keeping the Microscope Clean

General advice on maintaining the cleanliness of the microscope body.

Microscope Storage and Inspections

Proper Microscope Storage Guidelines

Recommendations for storing the microscope to prevent damage.

Regular Inspection and Maintenance

Importance of regular checks and maintenance for peak performance.

Troubleshooting Tables

Viewing and Control Systems Issues

Common problems related to image visibility, focus, and control functions.

Electrical System Troubleshooting

Specifications and Ratings

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