Nikon Eclipse E400 User Manual

ì
Ma
- Selecting a Condenser
Object!
Achromat/
Swi
Low-
bjective
aplanat
wing-out
Achromat
Abbe
magnification
magnification
condenser
condenser
condenser
condenser
condenser
1x
κ
κ
x
x
2x
x
x
x
©
(Note
2)
(Note
3)
4x
x
(Note
1)
(Note
1)
10x
to
100x
©
x
©
:
Optimum
:
Appropriate
X:
Inappropriate
Note 1: The entire field of view may not be covered when a UW eyepiece is used.
Note
2:
Indoor
lighting
and
similar
light
sources
may
be
reflected
off
the
surface
of
the
condenser
lens
and
be
visible
within
the
field
of
view.
When
this
happens,
either
dim
the
indoor
lighting
or
prevent
strong
outside
light
from
entering
onto
the
stage.
Note 3: Swing out the top lens.
@
Depending
on
the
type
of
objective,
the
whole
numerical
aperture
of
the
objective
may
not
be
covered.
For
example,
when
an
objective
with
N.A.
1.4
is
used
in
combination
with
a
swing-out
condenser
or
abbe
condenser,
the
aperture
size
will
only
be
about
65%
of
the
objective's
N.A.
even
when
it
is
opened
to
its
limit.
O
For
the
universal
system
condenser
and
phase
contrast
condensers,
refer
to
each
instruction
manual.
ο Oil-immersion operation
(using
an
oil-immersion
type
objective
and
condenser)
Objectives marked “Oil” are oil-immersion type objectives.
These objectives are used with the provided immersion oil
applied between the specimen and the tip of the objective.
In order to get maximum performance from oil-immersion type
objectives with a numerical aperture of 1.0 or greater, an oil-
immersion type achromatic aplanat condenser must also be used.
Oil-immersion type condensers are used with oil applied between
the specimen and the condenser lens.
Bubbles in the oil will adversely affect the viewing of the image.
Be
careful
to
prevent
the
formation
of
air
bubbles.
To
check
for
air
bubbles,
remove
the
eyepieces,
open
the
field
diaphragm
and
the
aperture
diaphragm
as
far
as
possible,
and
look
at
the
exit
pupil
of
the
objective
within
the
eyepiece
tube.
(The
exit
pupil
will
appear
as
a
bright
circle.)
If
it
is
difficult
to
see
if
there
are
any
bubbles,
mount
a
centering
telescope
(sold
separately)
on
an
eyepiece
sleeve
by
using
an
adapter
(sold
separately).
Then,
while
turning
the
eyepiece
on
the
centering
telescope
to
change
the
focus,
look
through
the
centering
telescope
for
air
bubbles.
If
there
are
bubbles
in
the
oil,
remove
them
by
one
of
the
following
methods:
Turn
the
revolving
nosepiece
slightly
to
move
the
oil-immersed
objective
back
and
forth
one
or
two
times.
(In
the
case
of
the
condenser,
gently
turn
the
condenser
focus
knob
to
move
the
condenser
up
and
down
slightly.)
©
Add
more
oil.
<>
Remove
the
oil
and
replace
it
with
new
oil.
A
©

Nikon Eclipse E400 Specifications

General

Type	Biological Microscope
Optical System	CFI60 Infinity Optical System
Nosepiece	Quadruple nosepiece
Magnification	40x to 1000x
Power Supply	AC 100-240V, 50/60Hz
Lamp Power	6V 30W
Eyepiece	10x
Objective Lenses	4x, 10x, 40x, 100x
Stage	Mechanical stage
Focus	Coaxial coarse and fine focusing
Condenser	Abbe condenser, N.A. 1.25
Observation Tube	Binocular
Stage Dimensions	134mm
Interpupillary Distance Adjustment	50-75mm
Diopter Adjustment	+/- 5 diopters
Illumination	Built-in Koehler illumination