Nikon Eclipse E400 - Condenser Installation and Alignment; Condenser Installation Procedure; Condenser Focusing and Centering Steps

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Installing
the
Condenser
O Installation
1-1
Turn
the
condenser
focus
knob
to
lower
the
condenser
holder
as
far
as
it
will
go.
1-2
Insert
the
condenser
in
the
condenser
holder.
1-3
Tighten
the
clamp
screw
on
the
left
side
with
the
aperture
scale
on
the
condenser
facing
to
the
front.
1-4
Turn
the
condenser
focus
knob
to
raise
the
condenser
as
far
as
it
will
go.
© Focusing and centering
Focus
and
center
the
condenser
so
that
the
light
passing
through
the
condenser
forms
the
image
at
the
proper
position
on
the
specimen (i.e., at the center of the optical path).
2-1
Focus
on
the
specimen
with
the
10x
objective.
2-2
Close
the
field
diaphragm
to
its
minimum
setting.
2-3
Turn
the
condenser
focus
knob
so
that
the
image
of
the
field
diaphragm
forms
on
the
specimen.
2-4
Adjust
condenser
centering
screws
so
that
the
image
of
the
field
diaphragm
appears
roughly
at
the
center
of
the
eyepiece
view field.
2-5
Move
the
40x
objective
into
the
optical
path.
Turn
the
fine
focus
knob
to
focus
on
the
specimen.
2-6
Turn
the
condenser
focus
knob
so
that
the
image
of
the
field
diaphragm
forms
on
the
specimen.
2-7
Adjust
the
condenser
centering
screws
so
that
the
image
of
the
field
diaphragm
appears
at
the
center
of
the
eyepiece
view
field.
This
adjustment
is
easier
to
make
if
you
adjust
the
size
of
the
field
diaphragm
to
slightly
smaller
than
the
eyepiece view field.