Chapter 4 Microscopy (Detailed Procedure)
57
4
6
Eyepiece Diopter Adjustments
Adjust the diopter ring on the eyepieces according
to the difference between your left and right
eyesight. This adjustment enables the user to take
full advantage of the high-quality objectives,
including their parfocality.
(1) Swing the 40x objective in the optical
path. Rotate the coarse and then fine
focus knobs to bring the specimen in
focus.
(2) Switch back to the 10x (or 4x) objective.
While looking into the right eyepiece
with your right eye, focus on the
specimen by rotating the right diopter
ring and not using the focus knob.
(3) While looking into the left eyepiece with
your left eye, focus on the specimen by
rotating the left diopter ring and not
using the focus knob.
(4) Repeat the steps c to e.
The next procedure is for microscopes equipped
with field diaphragm. If the microscope does
not have a field diaphragm, confirm the position
of the condenser and skip to procedure 8. (The
condenser should be a little lower than its
highest position.)
7
Field Diaphragm Centering and
Focusing (for microscopes with
the field diaphragm)
The field diaphragm is used to limit the specimen’s
illuminated area to the size of the viewfield. If
off-centered, its image will also be off-centered in
the viewfield especially when closed down. Follow
the steps below to match the centers of the
viewfield and the field diaphragm image.
(1) Swing the 10x (or 4x) objective in the
optical path. Close the field diaphragm to
its minimum aperture by rotating the
field diaphragm ring.
(Continued on the following page)
Focus with
this knob.
Use your
left eye.
Use your
right eye.
And focus with
this ring.
Focus.
Close down
the field
diaphragm.
Then focus
with this ring.
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