Olympus CH30 - Focusing and Immersion Objectives; Focusing Adjustment Knobs and Lever; Using Immersion Objectives

Fig. 25
CH30/CH40
© sin te Coarse Adjustment Knob Tension (ig.25)
1. Tho coarse adjustment knob tension is preadjusted for easy use.
However, if desired, one can change the tension using the tension
adjustment ring O. Applying a fla-bladed screwdriver to any of the
‘grooves @ on the circumference of the ring and turning the ring in
the direction of the arrow increases tension, and ice versa
2. Tho tension is too low the stage drops by seli or focus is quickly
lost after adjustment with the fine adjustment knob G. n this case,
tum the ring D in the direction of the arrow to increase tension
(EB Pre-focusing Lever (0.29)
The pre-focusing lever ensures that the objective does not come in
contact with the specimen and simplifies focusing, After focusing on
the specimen with the coarse adjustment knob, turn this lever ©
clockwise in the direction of the arrow to set a lower limit on coarse
adjustment movement
© Focusing with the fine adjustment knob is not affected by this pro-
focusing lever. Accordingly, after using the coarse adjustment knob
to lower the stage for changing specimens or applying immersion oil
{see Section 5-8), refocusing is easily accomplished by rotating the
coarse adjustment knob to reach the pre-focusing position, then
making fine adjustments with the fine adjustment knob.
# When not required, leave the pre-focusing lever unlocked.
Use of Immersion Objectives (Fig. 27)
1. Focus on the specimen with a low power objective.
2. Place a drop of immersion oil provided) onto the specimen at the area
to bo observed.
3. Turn the revolving nosepiece to engage the immersion objective,
then focus using the fine adjustment knob.
+ Since any bubbles in the oil wll impair the
tho oil is free of bubbles.
a. To check for bubbles, remove the eyepiece and fully open the
field and aperture iris diaphragms, then look at the exit pupil of
the objective inside the observation tube. (The pupil should
appear round and bright.)
b. To remove bubbles, rock the nosepiece slightly to move the oil
immersion objective back and forth a few times.
Q if the condenser marking shows a numerical aperture (NA) of 1.0 or
more, the number applies only when oil is present between the slide
glass and the top surface of the condenser. When oil is not present,
the NA is about 0.9.
4. After use, remove oil from the objective front lens by wiping with
‘gauze slightly moistened with an ether (7 parts) / alcohol (3 parts)
mixture or with EE-6310,
+ Using too much alcohol can dissolve the lens adhesive.
A Caution on Using the Immersion Oil:
if the immersion oil comes into contact with your eye or skin,
tely tako the following action.
For aye: Rinse with clean water (for more than 15 min).
For skin: Wash with soap and water.
ho appearance of the eye or skin changes or pain continues,
please consult your doctor.
rage, make sure that
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