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Olympus CKX41 - User Manual

Olympus CKX41
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INSTRUCTIONS
REFLECTED
FLUORESCENCE
SYSTEM
FOR CKX41
A X 7 3 8 9
This instruction manual is for the Olympus Reflected Fluorescence System for
use with the CKX41 culture microscope. To ensure the safety, obtain optimum
performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the system.
Retain this instruction manual in an easily accessible place near the work desk
for future reference.
Modules described in this manual
CKX-RFA
U-LH50HG
U-RFLT50
CKX-NU
This publication is printed on 100% recycled paper

Table of Contents

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Questions and Answers

  • L
    Lisa HillJul 26, 2025
    What to do if the main switch is on but the burner doesn't ignite on my Olympus Laboratory Equipment?
    • D
      Daniel ElliottJul 26, 2025
      If the main switch of your Olympus Laboratory Equipment can be set to ON, but the burner does not ignite, there are several possible reasons. First, ensure the lamp housing connecting cord is properly connected. Second, check if the mercury burner is mounted. If not, attach one. Third, the safety mechanism in the lamp housing might be activated; tighten the lamp burner socket clamping screw firmly. Lastly, the auto ignition system could be malfunctioning. Try setting the main switch of the power supply unit to OFF and then ON again.
  • D
    David FoxJul 30, 2025
    Why does the mercury burner flicker or have low brightness on my Olympus Laboratory Equipment?
    • I
      Isaac EsparzaJul 30, 2025
      If you're experiencing a flickering mercury burner or low brightness with your Olympus Laboratory Equipment, it could be due to several reasons. One possibility is that the auto ignition safety mechanism is activated. To resolve this, press the switch inside the starter reset hole on the right panel of the power supply unit, and then set the main switch to ON again. Another reason might be that you are observing this phenomenon shortly after ignition, in this case wait for 10 minutes or more after ignition. Ensure that the power supply voltage and frequency switches are set correctly. Finally, the burner life might have expired, requiring you to replace the mercury burner.
  • K
    Katie WestAug 3, 2025
    Why is the edge of the field of view obscured on my Olympus Laboratory Equipment?
    • P
      pricemeganAug 3, 2025
      If the edge of the field of view is obscured or unevenly illuminated on your Olympus Laboratory Equipment, consider the following: * Ensure the objective is properly engaged in the light path by making sure the revolving nosepiece clicks into place. * Verify the fluorescent mirror is correctly engaged in the light path. * Check that the filter slider is fully in the open position and not stopped halfway. * Confirm the ND filter is also fully engaged and not stopped halfway. * Adjust the centering of the mercury burner. * Adjust the collector focus position to an optimum setting.
  • T
    Tyler AguilarAug 8, 2025
    What to do if the main switch cannot supply power to my Olympus Laboratory Equipment?
    • M
      Michael HobbsAug 8, 2025
      If the main switch of your Olympus Laboratory Equipment cannot supply power to the system, it could be due to a couple of reasons. First, ensure that the power cord is properly connected. Second, check if a fuse is blown and replace it if necessary.
  • T
    Thomas MoranAug 12, 2025
    Why is the visibility poor and the image not sharp on my Olympus Laboratory Equipment?
    • R
      robert54Aug 12, 2025
      If you are experiencing poor visibility, a non-sharp image, or poor contrast with your Olympus Laboratory Equipment, there are a few potential causes. First, if you're using a 40X objective, it may be inaccurate, or the correction collar may not be properly adjusted; use the objective with correction collar and adjust it to find the best position. Second, the objective or filter might be dirty, so clean them thoroughly. Third, the field iris diaphragm may be adjusted improperly; adjust it so that it circumscribes the field of view.
  • S
    Stephanie LeeAug 16, 2025
    Why are the service lives of mercury burners very short in my Olympus Laboratory Equipment?
    • A
      Ashley PenningtonAug 16, 2025
      If the service lives of mercury burners are very short in your Olympus Laboratory Equipment, it might be because the power supply voltage is set improperly. Ensure that the power supply voltage is set correctly.
  • G
    greggrodriguezAug 21, 2025
    How to fix invisible or dark field of view with mercury burner on Olympus Laboratory Equipment?
    • N
      Nancy Daugherty MDAug 21, 2025
      If the field of view seen through the eye-pieces of your Olympus Laboratory Equipment is invisible or dark, even though the mercury burner illumination is on, there are a few possible reasons. Check if an ND filter is engaged in the light path and set the filter slider to the open position. Also, verify that the fluorescent mirror is properly engaged in the light path. Ensure that the shutter is not engaged in the light path and set the filter slider to the open position.

Summary

IMPORTANT - Be sure to read this first for safe use of the equipment.

Safety Precautions

Essential safety guidelines for operating the reflected fluorescence system.

Safety Symbols

Explains symbols used for safety on the equipment.

Warning Indications

Highlights critical warning positions on the system.

Getting Ready

Initial steps and precautions before system operation.

2 Maintenance and Storage

Caution

Explains the meaning of symbols and general cautions for system use.

1 NOMENCLATURE

2 CONTROLS OF EACH MODULE

Reflected Fluorescence Illuminator CKX-RFA

Details the controls and functions of the CKX-RFA illuminator.

Lamp Housing U-LH50 HG

Explains the controls for the lamp housing.

ND Filter 32 ND6;12;25;50

Describes the ND filter specifications.

Power Supply Unit for 50 W Mercury Burner U-RFLT50

Details the controls and features of the power supply unit.

3 SUMMARY OF REFLECTED FLUORESCENCE OBSERVATION PROCEDURE

Preparation

Steps and controls required before starting observation.

4 USING THE CONTROLS

1 General Precautions for Observation

Key precautions to ensure safe and optimal observation with the system.

Precautions on the specimen color fading

Advice on minimizing specimen color fading during fluorescence observation.

2 Selecting the Reflected Light Fluorescent Mirror

Information on choosing and using fluorescent mirrors for different excitations.

3 Applicable Fluorescence Objectives

Lists compatible objectives for fluorescence observation.

Applicable Fluorescence Objectives (continued)

Lists compatible objectives for fluorescence observation across different microscopy types.

4 Turning On the Mercury Burner

Step-by-step guide to safely ignite and operate the mercury burner.

5 Centering the Mercury Burner

Procedure for correctly aligning the mercury burner for optimal illumination.

6 Centering the Field Iris Diaphragm

How to adjust the field iris diaphragm for optimal contrast.

7 Switching the Filter Slider

Instructions for operating the filter slider for various functions.

5 TROUBLESHOOTING GUIDE

1. Optical System

Problems and solutions related to the optical components of the system.

2. Electrical System

Troubleshooting for electrical issues within the system.

Problem: Main switch can be set to ON but burner does not ignite

Identifies causes and remedies for burner ignition failures.

Problem: Mercury burner flickers or brightness is low

Addresses issues with burner flickering or inconsistent brightness.

Problem: Service lives of mercury burners are very short

Discusses causes for premature mercury burner expiration.

6 SPECIFICATIONS

7 ASSEMBLY

7-1 Assembly Diagram

Visual guide showing the order of assembling system components.

7-2 Detailed Assembly Procedure

Step-by-step instructions for assembling the reflected fluorescence system.

1 Removing the Dummy Cover

Instructions for removing the dummy cover during assembly.

2 Removing the Dust Cover and Fluorescent Mirror Slider

Steps to remove dust covers and the fluorescent mirror slider.

3 Attaching the CKX-RFA Reflected Fluorescence Illuminator

Guide for attaching the main fluorescence illuminator unit.

4 Attaching the Fluorescent Mirror Slider

How to re-attach the fluorescent mirror slider after removal.

5 Attaching the Dust Covers

Instructions for fitting the dust covers onto the system.

6 Attaching the Excitation Light Protective Shield

Steps for mounting the excitation light protective shield.

7 Attaching the U-LH50 HG Lamp Housing

Procedure for attaching the lamp housing to the illuminator.

8 Attaching the High-Pressure Mercury Burner

Detailed guide for installing the mercury burner into the lamp housing.

5. Set the burner type switch

Setting the burner type switch on the power supply unit.

Caution for burner replacement during observation

Important safety notes before replacing the burner.

6. Press the reset button

Resetting the hour counter after burner replacement.

8 U-EXCITATION FILTER SET CKX-NU (OPTIONAL)

Contents of the set

Lists the components included in the U-excitation filter set.

Construction of the fluorescent mirror slider

Details the construction of the fluorescent mirror slider assembly.

Removing the UV cut filter

Instructions for removing the UV cut filter from the lamp housing.

PROPER SELECTION OF THE POWER SUPPLY CORD

Specifications

Technical specifications for the power supply cord.

Table 1 Certified Cord

Lists certified agencies and their markings for power supply cords.

Table 2 HAR Flexible Cord

Details HAR flexible cord approval organizations and marking methods.

Olympus CKX41 Specifications

General IconGeneral
BrandOlympus
ModelCKX41
CategoryMicroscope
LanguageEnglish

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