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Olympus U-AN-2 - User Manual

Olympus U-AN-2
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INSTRUCTIONS
This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 8 8
BX3-URA
BX3-RFAS
U-LH100HGAPO
U-LH100HG
U-RFL-T
U-AN-2
BX3-25ND6
BX3-25ND25
REFLECTED
FLUORESCENCE
SYSTEM

Questions and Answers

  • K
    Kayla WilliamsAug 6, 2025
    Why can't I see anything through my Olympus U-AN-2, even though the burner is on?
    • J
      Joseph WestAug 6, 2025
      If the burner is on but the image from your Olympus Microscope is dark, first ensure the shutter is open. Then, check if the ND filter is engaged and remove it if necessary. Verify that the fluorescence mirror unit is correctly engaged and matches the specimen. Also, make sure that the aperture and field iris diaphragms are centered and fully enlarged.
  • B
    Benjamin MillerAug 11, 2025
    What to do if the field of view on my Olympus U-AN-2 Microscope is obscured?
    • D
      Dennis NovakAug 11, 2025
      If your Olympus Microscope's field of view is obscured, ensure the objective and fluorescence mirror unit are correctly engaged in the light path. The revolving nosepiece and ND slider should click into place properly. Also, check that the field iris diaphragm is not set too small; center the aperture iris diaphragm and field iris diaphragm, then fully enlarge the field iris diaphragm. Finally, ensure the mercury burner is properly centered.
  • B
    Brian OwensAug 16, 2025
    Why is the mercury burner flickering on my Olympus Microscope?
    • M
      Michele PhillipsAug 16, 2025
      If the mercury burner of your Olympus Microscope flickers or appears dark, wait at least 10 minutes after ignition for it to stabilize. If the issue persists and the hour counter indicates 300 hours (USH-103OL), replace the mercury burner. Also, ensure the burner is centered on the optical axis.
  • J
    johnsonmeganAug 21, 2025
    How to improve the image quality of my Olympus U-AN-2 Microscope?
    • J
      Jonathan MirandaAug 22, 2025
      If the image from your Olympus Microscope appears low quality, not sharp, or poor in contrast, start by thoroughly cleaning the objective and filter for any dirt or dust. Also, verify that the fluorescence mirror unit matches the specimen you are viewing.
  • A
    Ashlee McbrideAug 27, 2025
    What causes dark spots in the field of my Olympus Microscope?
    • P
      Philip FrankAug 27, 2025
      If your Olympus Microscope field contains dark, spot-like areas, the burner or the burner side of the collector lens may be dirty or dusty. Clean these components to resolve the issue.
  • D
    David BartonSep 1, 2025
    Why won't the mercury burner light on my Olympus U-AN-2?
    • R
      ryanwilliamSep 1, 2025
      If the mercury burner on your Olympus Microscope does not light even when the main switch is set to “I” (ON), first, ensure all connectors are firmly connected. Then, check that the mercury burner is properly attached. Verify that the safety device in the lamp housing is correctly set up. If auto ignition is malfunctioning, set the main switch of the power supply unit to “ ” (OFF) then “I” (ON) again, repeating as needed.

Summary

Important System Information

Safety Precautions

Getting Ready

Maintenance and Storage

Cautionary Notes

Nomenclature

Reflected Fluorescence Observation Procedure

Using the Controls

General Precautions for Observation

General precautions for safe and effective operation, including handling and specimen considerations.

Selecting the Fluorescence Mirror Unit

Guidance on choosing the appropriate fluorescence mirror unit based on excitation light bandwidth and fluorochrome.

Turning the Power Supply Unit On

Procedure for safely turning on the power supply unit and allowing the illumination to stabilize.

Centering the Field Iris Diaphragm

Step-by-step guide to centering the field iris diaphragm for optimal image contrast and reduced fading.

Centering the Aperture Iris Diaphragm (BX3-URA)

Procedure for centering the aperture iris diaphragm to adjust brightness and improve contrast.

Centering the Mercury Burner

Instructions for centering the mercury burner to ensure proper light incidence and prevent dark observation images.

Precise Centering of the Mirror

Procedure for precise mirror centering after mercury burner centering for optimal performance.

Mounting the ND Filters

Guidance on inserting and using Neutral Density (ND) filters to reduce excitation light intensity and prevent specimen fading.

Attaching the Light Shield Sheet

Instructions for attaching the light shield sheet to prevent fluorescence image degradation from reflected light.

Simultaneous Fluorescence Observations

Simultaneous Reflected Fluorescence and Phase Contrast Observations

Procedure for combining reflected fluorescence and phase contrast observation for enhanced specimen viewing.

Simultaneous Reflected Fluorescence and Transmitted Light DIC Observations

Steps for performing simultaneous reflected fluorescence and DIC observation for detailed specimen analysis.

Troubleshooting Guide

Optical System Issues

Troubleshooting for optical issues like no image, poor quality, or uneven illumination.

Electrical System Issues

Troubleshooting for electrical problems such as power issues or burner ignition failures.

Spectral Characteristics of Filters

Specifications

Assembly

Assembly Diagram

Visual guide illustrating the sequence of assembly for the various microscope modules and components.

Detailed Assembly Procedures

Step-by-step instructions for detailed assembly, including attaching fluorescence mirror units.

Attaching the Mercury Burner and Lamp Housing

Detailed steps for securely attaching the mercury burner and lamp housing to the microscope system.

Making an Optional Fluorescence Mirror Unit

Instructions on fabricating custom fluorescence mirror units using available filters and an empty unit frame.

Lamp Housing Inspection Sheet

Proper Selection of the Power Supply Cord

Olympus U-AN-2 Specifications

General IconGeneral
BrandOlympus
ModelU-AN-2
CategoryMicroscope
LanguageEnglish

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