To Next PageTo Next PageWhy does the Olympus U-LH100HGAPO field contain dark, spot-like areas?
If the field of your Olympus Microscope contains dark, spot-like areas, it is likely due to dirt or dust on the burner or on the burner side of the collector lens. Clean these components to resolve the issue.
Why is the field of view on my Olympus Microscope obscured?
If the field of view in your Olympus Microscope is obscured or not evenly illuminated, several factors could be the cause. First, ensure that the objective is correctly engaged in the light path and that the revolving nosepiece clicks correctly into place. Second, verify that the fluorescence mirror unit in use matches the specimen. Third, fully enlarge the field iris diaphragm. Fourth, make sure that the ND slider clicks properly into place. Finally, the mercury burner might not be centered, or the focusing could be defective; in this case, center the mercury burner or perform a focusing adjustment.
Why is the mercury burner on my Olympus U-LH100HGAPO Microscope flickering?
If the mercury burner in your Olympus Microscope is flickering or appears dark, it could be due to several reasons. If it is soon after ignition, allow it to run for at least 10 minutes. If the burner life has expired, and the hour counter indicates 200 hours (USH102D) or 300 hours (HBO103W/2), replace it. Also, the burner might be deviated from the optical axis, so you should center the mercury burner.
How to fix a dark field of view on an Olympus Microscope?
If the bulb lights up but the field of view remains dark on your Olympus Microscope, ensure that the shutter is released. Also, check if the ND filter is engaged in the light path and disengage it if necessary. Verify that the fluorescence mirror unit is correctly engaged in the light path and that the aperture and field iris diaphragms are fully enlarged until they circumscribe the field of view. Finally, confirm that the fluorescence mirror unit in use matches the specimen.
What to do if the Olympus U-LH100HGAPO Microscope turns on but the mercury burner won't light?
If the main switch of your Olympus Microscope turns the system on, but the mercury burner does not light, check that the connectors are firmly connected. Ensure the mercury burner is attached. Verify that the safety device in the lamp housing is correctly set up. If auto ignition is malfunctioning, set the main switch of the power supply unit to “ ” (OFF) then “ I ” (ON) again; repeat if necessary.
Why is the image on my Olympus U-LH100HGAPO Microscope low quality, not sharp, or poor in contrast?
If the image from your Olympus Microscope is low quality, not sharp, or poor in contrast, the following steps may help: Clean the objective or filter thoroughly to remove any dirt or dust. Fully enlarge the aperture iris diaphragm and enlarge the field iris diaphragm until it circumscribes the field of view. Ensure that the fluorescence mirror unit matches the specimen being viewed.
Why is the Olympus U-LH100HGAPO burner on, but the light is dark or not visible from the eyepiece?
If the burner is ON but the light cannot be seen from the eyepiece or is dark, check the following: First, open the shutter. Second, remove the ND filter if it is engaged in the light path. Third, ensure the fluorescence mirror unit is correctly engaged in the light path. Fourth, fully enlarge the aperture iris diaphragm and enlarge the field iris diaphragm until it circumscribes the field of view. Finally, make sure the fluorescence mirror unit matches the specimen.
How to fix an obscured or unevenly illuminated field of view on an Olympus U-LH100HGAPO?
If the field of view of your Olympus Microscope is obscured or not evenly illuminated, consider these potential causes: Ensure the objective is correctly engaged in the light path by making sure the revolving nosepiece clicks properly into place. Verify that the fluorescence mirror unit is correctly engaged in the light path. Fully enlarge the field iris diaphragm. Confirm that the ND slider is stopped at the click position. Center the mercury burner or perform a focusing adjustment.
What to do if the field of view on my Olympus Microscope is obscured?
If your Olympus Microscope's field of view is obscured, ensure the objective and fluorescence mirror unit are correctly engaged in the light path. The revolving nosepiece and ND slider should click into place properly. Also, check that the field iris diaphragm is not set too small; center the aperture iris diaphragm and field iris diaphragm, then fully enlarge the field iris diaphragm. Finally, ensure the mercury burner is properly centered.
Why won't the turret rotate on my Olympus U-LH100HGAPO Microscope?
If the turret on your Olympus Microscope will not rotate, or stops in the middle of movement, it may be because the fluorescence mirror unit is slipping out of the turret. Remove the turret and place the fluorescence mirror unit in the correct position securely.
General| Brand | Olympus |
|---|---|
| Model | U-LH100HGAPO |
| Category | Microscope |
| Language | English |
Essential safety guidelines for operating the system and handling its components.
Identification and labeling of the system's key parts.
Step-by-step instructions for conducting reflected fluorescence observations.
Guide to operating the various controls for optimal system performance.
Procedures for combining fluorescence observation with other microscopy techniques.
Solutions for common problems encountered during system operation.
Graphs and data showing the spectral properties of system filters.
Overview of system setup for reflected observations on BX-URA2.
Instructions for assembling the reflected observation system components.
Warnings regarding the hot mercury burner and lamp housing during and after operation.
Precautions for handling the power supply unit, including voltage and grounding.
General guidelines for observation, including power supply and illumination.
Guidance on selecting the appropriate fluorescence mirror unit based on fluorochrome characteristics.
Table detailing objective compatibility for different observation modes.
Details on mirror and filter combinations for various excitation methods.
Filter configurations specifically for fluorescent protein applications.
Procedure for powering on the system's power supply unit.
Steps for aligning the field iris diaphragm for optimal illumination.
Steps for aligning the aperture iris diaphragm for optimal contrast.
Procedure for centering the mercury burner for consistent illumination.
Instructions on how to mount Neutral Density (ND) filters.
Procedure for combined reflected fluorescence and phase contrast observation.
Procedure for combined reflected fluorescence and DIC observation.
Troubleshooting common issues related to the optical components of the system.
Troubleshooting common issues related to the electrical components of the system.
Description and mounting procedure for the 6-position filter slider.
Description and mounting procedure for the 6-position barrier filter slider.
Adjusting field and aperture diaphragms for reflected observation.
Different types of observations for the reflected system.
Optical properties of UIS2/UIS objectives for reflected light.
Troubleshooting common issues with the reflected observation system.
Visual guide showing the sequence of assembly for microscope modules.
Step-by-step instructions for assembling the various system components.
