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D. Chemical Compatibility / Solvent Rinsing of Zebron
™
Capillary Columns
Important! Water and organic solvents such as those listed in Table 1 will not damage Zebron column stationary phases. However, inorganic acids and bases should
be completely avoided or rapid degradation and permanent damage to the stationary phase will result. In the event chemical damage is incurred, the removal of 0.5-2
meter of capillary off the front end will often restore column performance. Select the rinse solvent from the Table below.
Table 1. Phase Compatibility with Rinsing Solvents
Table 2. Rinsing Conditions
Column ID (mm) Rinse Solvent Volume (mL) Pressure (psig)
0.25 5 40
0.32 5 40
0.53 10 20
Important: Rinse from the back to the front of the column to avoid pushing inlet contaminants further into the column.
Phase Water Methanol CH
2
Cl
2
CHCl
3
Acetone Hexane
ZB-1
ZB-1ms
ZB-1HT Inferno
™
ZB-5
ZB-5ms
ZB-5MSi
ZB-5HT Inferno
™
ZB-XLB
ZB-624
ZB-35
ZB-1701
ZB-1701P
ZB-50
ZB-WAX
PLUS
™
AVOID
ZB-WAX AVOID
ZB-FFAP AVOID
MR-1
MR-2
ZB-Drug-1
ZB-BAC1
ZB-BAC2
ZB-Bioethanol
OK
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