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3
A. Column Conditioning
Allow sufficient time for the carrier gas to flow through the column to 1.
purge any oxygen that may be in the system.
Raise the temperature of the column to the maximum isothermal 2.
operating temperature that is listed on the individual Zebron
™
GC
Column Test Report. Maintain this temperature until a constant baseline
is achieved. Conditioning times will depend on the phase identity and
thickness, with thicker films taking longer to stabilize. If necessary
in order to minimize the downtime of the instrument, columns can be
conditioned overnight at the maximum isothermal temperature.
B. Installation Testing
Inject a detectable unretained sample, such as methane for an FID, to determine
dead volume time and linear gas velocity at the desired column temperature.
Adjust gas pressure for optimal flow depending on carrier gas selection.
The non-retained peak must have ideal peak shape or installation is faulty 1.
and needs to be redone.
C. Checking for Leaks
Use a thermoconductivity detector to check for leaks. It is highly sensitive to
H
2
, He, and N
2
and will not contaminate the instrument or column. Liquid leak
indicators are not recommended for capillary columns. There is the risk of
drawing the liquid into the column or fittings and contaminating the system.
NOTE: If Vespel
®
ferrules are being used, leakage can occur after the initial
heating phase due to ferrule deformation. Be sure that the fitting is re-tightened
after this initial heating phase, and then carefully check all corrections for leaks.
D. Test Column with Zebron’s Text Mix
Good chromatographic practices include the use of Performance Check
Standards to establish the baseline performance characteristics of your column
and to monitor any changes over time. Test solutions should evaluate column
inertness, efficiency and resolution in an easy-to-use format. Most Phenomenex
test mixes are supplied ready to use at a concentration of 250 μg/mL in glass
ampules.
Match your column phase to the correct standard to set up initial running
conditions. Should you have any questions on the proper use of these Check
Standards, contact your local Phenomenex GC Specialists.
Zebron’s Text Mix
Suitable for Phenomenex Zebron•
™
and equivalent brands
Convenient way to check column performance•
Affordable and easy-to-use•
Zebron Phase Test Mix
Part Number
ZB-1 AG0-5155
ZB-1ms AG0-7805
ZB-1HT AG0-5155
ZB-5 AG0-5155
ZB-5ms AG0-7578
ZB-5MSi AG0-8362
ZB-5HT AG0-5155
ZB-35 AG0-5156
ZB-1701 AG0-5156
ZB-1710P AG0-5156
ZB-50 AG0-5157
ZB-624 AG0-5159
ZB-WAX AG0-5158
ZB-WAXPLUS
™
AG0-7869
ZB-FFAP AG0-5158
ZB-XLB AG0-7578
ZB-Drug-1 AG0-8431
Grob Test AG0-5154
GC Column Conditioning and Testing
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