REPLIGEN XCell ATF10 User Manual

XCell
TM
ATF System with C410:V4B Controller User Guide
77
System-stop
If the XCell™ ATF is stopped by the System-stop (one of 2 blue buttons on either the P-Box or E-
Box), and the System-stop is reset, the XCell™ ATF will not restart until the master has to reset
Start/stop to 0 and then set back to 1. System-stop is indicated visually with the buttons
illuminated and indicated via profibus by OutputPage0.System Stop Active (%DB31.DBX366.5)
Calibration Process
When not running, calibration activities can be performed.
The master sends input parameters pages 3 and 4 to XCell™ ATF or calibrate locally. As in
Remote Set-up, the Start/Stop parameter needs to be “0”.
Repligen recommends having a service technician perform calibration activities. Once loop
calibration is initiated, it must complete the calibration process. There is currently no means to
disable an enabled calibration without completing the process via remote commands.
Only one input loop shall be calibrated at a time. Each loop shall be calibrated in its entirety by
itself, without enabling (or initiating) the calibration of another loop.
The process of calibration is a 2-point linear scaling process, where a technician first confirms all
required utilities, external pressure gauge and loop wiring is connected. The following steps are
performed:
1) An enable bit is momentarily asserted. Controller is enabled in calibration mode for a
particular analog input.
2) Pneumatic devices are set to produce a minimum setting. The pressure in the system
approaches the minimum value commanded. Pressure gauge reading is allowed to
stabilize.
3) The pressure gauge reading is provided to the controller. A minimum capture bit is
momentarily asserted.
4) Pneumatic devices are set to produce a maximum setting. The value on the pressure
gauge is allowed to stabilize.
5) The pressure gauge reading is provided to the controller. A maximum capture bit is
momentarily asserted.
6) Once both minimum and maximum points are captured, the linear scaling calculation
can be performed by the PLC. This calculation is initiated by setting an accept bit
(momentarily asserted). Controller performs the linear scaling calculation and resets
the controller’s internal calibration enable bit.
Calibration Example
Technician calibrates the P2 pressure sensor.
1) Assert enable bit:
a. Set “SendingDataPage” = 3
b. Set InputPage3.P2_Cal_Enable = 1 until the OutputPage4.P2_Cal_Enable bit is
received.
i. Wait for the “RequestedDataPage” to be equal to 4; since cycling through output
data pages is recommended.
ii. Examine OutputPage4.P2_Cal_Enable. When true…
c. Reset InputPage3.P2_Cal_Enable = 0.
d. Controller is enabled in calibration mode for P2 analog input.

REPLIGEN XCell ATF10 Specifications

General

Brand	REPLIGEN
Model	XCell ATF10
Category	Laboratory Equipment
Language	English

Summary

XCell™ ATF System

Description of the XCell™ ATF System and Process

XCell™ ATF System Pump Cycle

Details the operational cycle of the XCell™ ATF system pump.

XCell™ ATF System Control Scope and Objectives

Outlines the control capabilities and goals of the XCell™ ATF system.

XCell™ C410v4B Controller Layout

Filtration Assembly (Stainless Steel)

Details the stainless steel filtration assembly components and connections.

Pneumatics Box (P-Box) Components

Lists and describes the components within the Pneumatics Box (P-Box).

Electrical Box (E-Box)

Details the components housed within the Electrical Box (E-Box).

Power Box (Power Separation) Components

Describes the components located in the Power Box for power separation.

Primary Pneumatic Services

Explains the primary pneumatic connections and services for the system.

Primary Electric Services

Details the primary electrical connections and services required for operation.

C410v4B Controller Process and Control

Control Overview

Provides a general overview of the process and control mechanisms.

Control Functional Algorithms

Explains the algorithms used by the controller for process management.

Interface and Screens

Describes the user interface and various screens of the controller.

Startup

Guides users through the initial setup and startup procedures for the system.

XCell™ ATF Hollow Fiber Module and Diaphragm Replacement

Sterilization

Preparation of Filtration Assembly for Autoclaving

Steps for preparing the filtration assembly before autoclaving.

Autoclave Cycle

Details the recommended autoclave cycle for sterilization.

Connection to Bioreactor (ATF-to-Bioreactor)

Hard Connection

Describes the procedure for making a hard connection to the bioreactor, including SIP.

Maintenance

Diaphragm

Recommendations for diaphragm usage, orientation, and lifespan.

Appendix 2: Access levels to the C410v4B controller

Operators

Engineers

Administrators

Appendix 4: Profinet Communication

Profienet® Infrastructure

Configuration

Appendix 5: Profibus Communication

Remote Control Mode

Input Signals (from Master to XCell™ ATF)

Output Signals (to Master from XCell™ ATF)

OPC Configuration for DCS Integration

Black-Box Configuration

Prerequisites

Appendix 6: Delta V Example configuration

Install the GSD File in Delta V Explorer

Add new Profibus Device

Select Profibus Device Type

Address Setting

Confirm and Select “OK” button

Verify “Enabled”

Create Slot

Appendix 7: OPC Communication

XCell™ ATF Operations- Remote Monitoring and Data Acquisition

Appendix 8: Audit Trail (If Equipped)