REPLIGEN KrosFlo KR2i

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DOC0304 eRev. [Major Version]1.01.0 [Publication Date]8/9/20238/9/2023
KrosFlo® KR2i
RPM™ System
User Guide

REPLIGEN KrosFlo KR2i Specifications

General

Type	Tangential Flow Filtration System
Pump Type	Peristaltic
Control System	Touchscreen Interface
Connectivity	USB, Ethernet
Power Requirements	100-240 V AC, 50/60 Hz
Max Flow Rate	2 L/min
Weight	15 kg
Automation	Yes, programmable protocols
Operating Pressure	6 bar (87 psi)

Summary

Introduction

About this document

Safety Precautions

System Specifications

Computer

Details about the computer used with the system.

System Configuration and Major Components

Pump Drive & Integrated Pressure Monitor

Details about the pump drive and integrated pressure monitor.

Octopus Cables

Information on Octopus cables for system connectivity.

Mounting Hardware

Details on mounting hardware for the system.

Materials of Construction

Setup and Operation

Basic Setup

Step-by-step guide for initial system setup.

Manual Mode Setup

Configuration for operating the system in manual mode.

Concentration Mode (C. Mode) Setup

Setup procedures for concentration mode operation.

CD and CDC Mode Setup

Setup for C/D and C/D/C operating modes.

CDDC Mode Setup

Setup procedures for the C/D/D/C operating mode.

CFC Mode Setup

Setup configuration for CFC mode operation.

CFDC Mode Setup

Setup configuration for CF/D/C mode operation.

System Setup Using KrosFlo RPM Software Pump Control Window

Using the software control window for system setup.

Alarms and Stops

Configuration and types of safety alarms and stops.

Tubing Calibration

Procedure for calibrating tubing within the pump head.

Pump Head Setup and Operation

KR2 i Installation and Removal

Instructions for installing and removing the KR2i pump head.

KR2 i Tubing

Instructions for loading and unloading tubing in the KR2i pump head.

KR2 i Multi-Channel

Instructions for mounting multiple pump heads in tandem.

KR2 i Maintenance

Cleaning and maintenance guidelines for the KR2i pump head.

FlowVPX Instrument Setup

Installation and Basic Setup

Initial installation and basic setup of the FlowVPX instrument.

Connecting the FlowVPX Head to the FlowVPX Standard Mount

Steps to connect the FlowVPX Head to its standard mount.

Connecting the FlowVPX Instrument to the Cary 60 Spectrophotometer and Computer

Connecting the FlowVPX to the spectrophotometer and computer.

Loading and Unloading the Flow Cell

Procedure for loading and unloading the flow cell.

Unloading the Flow Cell

Procedure for unloading the flow cell from the instrument.

SoftwareFirmware Operation

Information on operating the system's software and firmware.

Auxiliary Component Setup and Operation

Auxiliary Scales

Setup and operation of auxiliary scale components.

Auxiliary Pumps

Setup and operation of auxiliary peristaltic pumps.

Auxiliary Pump Settings

Configuration settings for auxiliary pumps.

Automatic Backpressure Valve

Setup and operation of the automatic backpressure valve.

KONDUIT

Setup and configuration of the KONDUIT module.

Basic Concepts of Tangential Flow Filtration

Concentration

Explanation of the concentration process in TFF.

Diafiltration

Explanation of the diafiltration process in TFF.

Troubleshooting

Replacement and Auxiliary Parts