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REPLIGEN XCell ATF Series

REPLIGEN XCell ATF Series
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XCell
TM
ATF System
With C410:V4B Controller
USER GUIDE
XCell
ATF4 | XCell
ATF6 | XCell
ATF10

Table of Contents

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REPLIGEN XCell ATF Series Specifications

General IconGeneral
Filtration MethodTangential Flow Filtration (TFF)
TypeXCell ATF System
ApplicationCell Culture Harvest, Clarification, Concentration, Diafiltration
TechnologyAlternating Tangential Flow (ATF)
ApplicationsCell Therapy, Vaccine Production
Volume Range1 L to 2000 L
Membrane Area0.1 m² to 10 m²
CompatibilityCompatible with various cell types and media
DimensionsVaries by model
WeightVaries by model
Power Requirements100-240 VAC, 50/60 Hz

Summary

1.0 Description of the XCell ATF System and Process

1.1 XCell ATF System Pump Cycle

Explains the alternating tangential flow generated by the diaphragm pump.

1.2 XCell ATF System Control Scope and Objectives

Outlines the functionalities and goals of the C410v4B controller.

2.0 Utility Requirements

3.0 Dimensions and Weight

4.0 XCell ATF Pump Housing Assemblies

5.0 XCell C410 v4 B Controller Layout

5.1 General Layout

Describes the three main components of the controller system.

5.2 Filtration Assembly (Stainless Steel)

Details the stainless steel filtration assembly components.

5.3 Pneumatics Box (P-Box) Components

Lists the components and connections within the Pneumatics Box.

5.4 Electrical Box (E-Box)

Details the HMI and PLC components within the Electrical Box.

5.5 Power Box (Power Separation) Components

Lists the components found in the Power Box.

5.6 Primary Pneumatic Services

Explains the air inlet and exhaust/vacuum line connections.

5.7 Primary Electric Services

Details power connection, switch, and stop button.

5.8 Signal

Describes sensor inputs, Ethernet/Profibus, and relays.

6.0 C410 v4 B Controller Process and Control

6.1 Control Overview

Explains how the controller manages diaphragm pump operation and flow.

6.2 Control Functional Algorithms

Details the algorithms used for diaphragm pump cycle control.

6.3 Interface and Screens

Describes the HMI interface, primary screens, and their functions.

6.4 Startup

Provides a step-by-step guide for initial system setup and operation.

6.5 Examples

Offers guidelines for optimizing operating conditions with practical examples.

7.0 XCell ATF Hollow Fiber Module and Diaphragm Replacement

7.1 Separating the Filtration Assembly from the bioreactor

Details the procedure for disconnecting the assembly from the bioreactor.

7.2 HFM removal

Describes the steps for removing the Hollow Fiber Module.

7.3 HFM insertion

Explains the process for inserting a new Hollow Fiber Module.

7.4 Screen Module replacement

Provides instructions for replacing a screen module.

7.5 Diaphragm replacement

Outlines the procedure for replacing the diaphragm in the pump.

8.0 Sterilization

8.1 Preparation of Filtration Assembly for Autoclaving

Details pre-autoclaving checks and assembly preparation.

8.2 Autoclave Cycle

Describes recommended autoclave cycle parameters and procedures.

8.3 Sterilization of Filtrate Harvest line

Explains the sterilization process for the filtrate/harvest line.

9.0 Connection to Bioreactor (ATF-to-Bioreactor)

9.1 Hard Connection

Provides the procedure for making a hard, steam-sterilizable connection.

10.0 Maintenance

10.1 Diaphragm

Recommends diaphragm replacement and proper seating.

10.2 Pump Air Inlet Filter

Advises on the replacement schedule for the air inlet filter.

10.3 O Rings, gaskets and Quick Connects

Specifies replacement intervals for O-rings and gaskets.

Appendix 1: Cycle time vs. Flow rate

Appendix 2: Access levels to the C410 v4 B controller

Appendix 4: Profinet Communication

Appendix 5: Profibus Communication

Appendix 6: Delta V Example configuration

Appendix 7: OPC Communication

Appendix 9: Example EU Declaration of Conformity

Appendix 10: UL Listing

Appendix 11: Spare Parts List

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