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REPLIGEN Spectrum KR2i - User Manual

REPLIGEN Spectrum KR2i
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Spectrum® KR2i
/ KMPi TFF Systems
USER GUIDE AND OPERATING INSTRUCTIONS
Spectrum® KrosFlo® Research 2i TFF System
Spectrum® KrosFlo® MiniKros Pilot i TFF System

Table of Contents

Questions and Answers

  • J
    Julian MartinezAug 3, 2025
    Why is my REPLIGEN Spectrum KR2i process ending early or late in DV or CF auto modes?
    • M
      michelle69Aug 3, 2025
      If your REPLIGEN Laboratory Equipment process is ending prematurely or belatedly in DV or CF auto modes, the input for the holdup volumes may be incorrect. Ensure you enter the correct feed holdup and permeate holdup volumes, and accurately specify whether they are empty or full when initiating the process.
  • M
    mary20Aug 11, 2025
    What to do if REPLIGEN Spectrum KR2i Laboratory Equipment process ends early when using Conductivity or UV for auto modes?
    • A
      Alexandra ChangAug 11, 2025
      If the REPLIGEN Laboratory Equipment process terminates prematurely when utilizing Conductivity or UV for auto modes, it may be due to the sensor not being completely filled with the appropriate liquid. The conductivity sensor should be filled with the same buffer as the sample at the start of the process. Likewise, the UV sensor should be zeroed using the same buffer the sample is in.
  • K
    Kelly HernandezAug 22, 2025
    How to fix wrong pressure sensor readings on REPLIGEN Spectrum KR2i?
    • F
      Frank BoltonAug 22, 2025
      If the pressure sensor readings on your REPLIGEN Laboratory Equipment are incorrect, displaying either -9.9 psi or >35psi when there is no pressure applied, it may indicate a malfunctioning pressure sensor octopus cable or a broken pressure sensor. You should replace the Octopus Cable and/or the pressure sensor.

Summary

Introduction to TFF Systems

Safety Precautions and Symbols

Explanation of Symbols

Defines hazard and safety symbols used throughout the manual for risk identification.

TFF System Specifications

KR2 i Specifications

Detailed technical parameters and performance specifications for the KR2i system.

KMPi Specifications

Detailed technical parameters and performance specifications for the KMPi system.

KONDUIT Specifications

Technical details for the KONDUIT accessory, including environmental and compliance data.

System Configuration and Components

Octopus Cables

Explanation of the function and connection of Octopus Cables for system components.

System Parts List

Materials of Construction

Setup and Operation Guide

Basic Setup

Initial steps for setting up the TFF system, including connecting components.

Manual Mode Setup

Step-by-step instructions for configuring the system in Manual Mode.

System Setup Using KF Comm Pump Control Window

Guide for configuring TFF system settings via the KF Comm software interface.

Hardware Setup

Procedures for configuring hardware parameters and system settings.

System Functionality Overview

Pump Controls

Details on controlling the TFF system's pump functions and modes.

Overview of Pump Controls

General overview of TFF system functions, pump modes, and auxiliary actions.

Detailed Pump Mode Operation

In-depth explanations of how to operate various TFF pump modes.

Running the TFF System

Procedures for starting and running a TFF system application.

Pump Head Setup and Operation

Specifications

Technical specifications for KR2i and KMPi pump heads and associated tubing.

KR2 i Pump Head Operation

Step-by-step instructions for installing and operating KR2i pump heads.

KMPi Pump Head Operation

Instructions for installing and operating KMPi pump heads.

Auxiliary Component Setup and Operation

Auxiliary Scales

Guide for installing and connecting auxiliary scales to the TFF system.

Auxiliary Pumps

Instructions for installing and configuring auxiliary peristaltic pumps.

Automatic Backpressure Valve

Setup, operation, and settings for automatic backpressure valves.

KF Comm Software Guide

Introduction to KF Comm Software

Overview of the KF Comm Software Suite and its compatibility.

Installing and Updating Software and Firmware

Guide for installing and updating TFF system software and firmware.

Data Collection Tools

Overview of the tools available within KF Comm for data collection.

Basic Concepts of Tangential Flow Filtration

Concentration Process

Explanation of the concentration principle in Tangential Flow Filtration.

Diafiltration Process

Explanation of the diafiltration process and its role in TFF.

Troubleshooting Common Issues

Replacement and Auxiliary Parts

REPLIGEN Spectrum KR2i Specifications

General IconGeneral
BrandREPLIGEN
ModelSpectrum KR2i
CategoryLaboratory Equipment
LanguageEnglish

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